- 700178 - Invitrogen Antibodies CLDN18 antibody

Submitted references Functional Analysis of Gastric Tight Junction Proteins in Xenopus laevis Oocytes.

Stein L, Brunner N, Amasheh S
Membranes 2022 Jul 23;12(8)

Transcriptomic Analysis of Testicular Gene Expression in a Dog Model of Experimentally Induced Cryptorchidism.

Jhun H, Lee WY, Park JK, Hwang SG, Park HJ
Cells 2022 Aug 10;11(16)

Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.

Lee-Thacker S, Jeon H, Choi Y, Taniuchi I, Takarada T, Yoneda Y, Ko C, Jo M
Scientific reports 2020 Jun 18;10(1):9921

Clinical Significance of CLDN18.2 Expression in Metastatic Diffuse-Type Gastric Cancer.

Kim SR, Shin K, Park JM, Lee HH, Song KY, Lee SH, Kim B, Kim SY, Seo J, Kim JO, Roh SY, Kim IH
Journal of gastric cancer 2020 Dec;20(4):408-420

Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis.

Quilichini E, Fabre M, Dirami T, Stedman A, De Vas M, Ozguc O, Pasek RC, Cereghini S, Morillon L, Guerra C, Couvelard A, Gannon M, Haumaitre C
Cellular and molecular gastroenterology and hepatology 2019;8(3):487-511

Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.

Weber B, Mendler MR, Lackner I, von Zelewski A, Höfler S, Baur M, Braun CK, Hummler H, Schwarz S, Pressmar J, Kalbitz M
PloS one 2019;14(7):e0219211

Prevention and Reversion of Pancreatic Tumorigenesis through a Differentiation-Based Mechanism.

Krah NM, Narayanan SM, Yugawa DE, Straley JA, Wright CVE, MacDonald RJ, Murtaugh LC
Developmental cell 2019 Sep 23;50(6):744-754.e4

Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.

Chen H, Lu R, Zhang YG, Sun J
Tissue barriers 2018;6(4):1-13

Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.

Zhou B, Flodby P, Luo J, Castillo DR, Liu Y, Yu FX, McConnell A, Varghese B, Li G, Chimge NO, Sunohara M, Koss MN, Elatre W, Conti P, Liebler JM, Yang C, Marconett CN, Laird-Offringa IA, Minoo P, Guan K, Stripp BR, Crandall ED, Borok Z
The Journal of clinical investigation 2018 Mar 1;128(3):970-984

CLDN18.1 attenuates malignancy and related signaling pathways of lung adenocarcinoma in vivo and in vitro.

Luo J, Chimge NO, Zhou B, Flodby P, Castaldi A, Firth AL, Liu Y, Wang H, Yang C, Marconett CN, Crandall ED, Offringa IA, Frenkel B, Borok Z
International journal of cancer 2018 Dec 15;143(12):3169-3180

NF-κB inhibitors impair lung epithelial tight junctions in the absence of inflammation.

Ward C, Schlingmann B, Stecenko AA, Guidot DM, Koval M
Tissue barriers 2015;3(1-2):e982424

The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function.

Overgaard CE, Schlingmann B, Dorsainvil White S, Ward C, Fan X, Swarnakar S, Brown LA, Guidot DM, Koval M
American journal of physiology. Lung cellular and molecular physiology 2015 Jun 15;308(12):L1212-23

The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma.

Krah NM, De La O JP, Swift GH, Hoang CQ, Willet SG, Chen Pan F, Cash GM, Bronner MP, Wright CV, MacDonald RJ, Murtaugh LC
eLife 2015 Jul 7;4

CD4+ T lymphocyte ablation prevents pancreatic carcinogenesis in mice.

Zhang Y, Yan W, Mathew E, Bednar F, Wan S, Collins MA, Evans RA, Welling TH, Vonderheide RH, di Magliano MP
Cancer immunology research 2014 May;2(5):423-35

Primary gastric Merkel cell carcinoma harboring DNA polyomavirus: first description of an unusual high-grade neuroendocrine carcinoma.

Capella C, Marando A, Longhi E, Bernasconi B, Finzi G, Parravicini C, Sessa F, La Rosa S
Human pathology 2014 Jun;45(6):1310-4

Distribution of LGR5+ cells and associated implications during the early stage of gastric tumorigenesis.

Jang BG, Lee BL, Kim WH
PloS one 2013;8(12):e82390

Linear and micronodular neuroendocrine cell hyperplasia in an ovarian mucinous cystadenoma.

Dainese E, Cimetti L, Capella C, Riva C
Pathology, research and practice 2013 Oct;209(10):670-3

Interleukin-6 is required for pancreatic cancer progression by promoting MAPK signaling activation and oxidative stress resistance.

Zhang Y, Yan W, Collins MA, Bednar F, Rakshit S, Zetter BR, Stanger BZ, Chung I, Rhim AD, di Magliano MP
Cancer research 2013 Oct 15;73(20):6359-74

Oncogenic Kras is required for both the initiation and maintenance of pancreatic cancer in mice.

Collins MA, Bednar F, Zhang Y, Brisset JC, Galbán S, Galbán CJ, Rakshit S, Flannagan KS, Adsay NV, Pasca di Magliano M
The Journal of clinical investigation 2012 Feb;122(2):639-53

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Claudin-18 was performed by loading 30 µg of rat Heart, A431 and A549 lysates using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. Claudin-18 was detected at ~27 kDa using Claudin-18 Recombinant Rabbit Monoclonal Antibody (Product # 700178) at a 1:1000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody (Product # G-21234) at a 1:5000 dilution and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Claudin-18 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Claudin-18 Recombinant Rabbit Monoclonal Antibody (Product # 700178) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c is a merged image showing cell junction localization and panel d is a no primary antibody control. The images were captured using a Nikon microscope at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of Claudin-18 was performed on paraffin-embedded human normal stomach and squamous lung carcinoma tissue sections. To expose target proteins, endogenous peroxide blocking followed by heat induced epitope retrieval was performed using Tris-EDTA (pH 9. 0) buffer for 15 minutes. Following antigen retrieval, tissues were blocked in 0. 2% BSA with 0. 1% cold fish skin gelatin in 1X PBS for 1 hour in a humidified chamber. The tissues were then probed with a Claudin-18 Recombinant Rabbit Monoclonal Antibody (Product # 700178) at a dilution of 2 µg/mL for 3 hours at room temperature in a humidified chamber. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody (Product # G-21234), followed by colorimetric detection using DAB. Images were taken at 40x (normal stomach) or 20x (lung carcinoma) magnification. Note membrane staining of normal stomach and cytoplasmic staining of lung carcinoma.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3--figure supplement 1. Microenvironmental remodeling in Ptf1a cKO; Kras G12D pancreata. Pancreata were harvested 2 weeks following TM administration (0.17 mg/g) to mice of the indicated genotypes. ( A - D ) Immunofluorescence for the PanIN marker CLDN18 (red), and the leukocyte marker CD45 (green), revealing association of leukocytes with PanINs in Ptf1a cKO; Kras G12D . ( E - H ) IHC for alpha-SMA, highlighting the activation of pancreatic stellate cells in Ptf1a cKO; Kras G12D pancreata. ( I - L ) Sirius Red staining, highlighting widespread fibrosis in Ptf1a cKO; Kras G12D . Scale bars: ( A - D ) 50 mum, ( E - L ) 200 mum. DOI: http://dx.doi.org/

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2. Loss of Ptf1a promotes acinar-to-ductal metaplasia and sensitizes acinar cells to KRAS-mediated transformation. ( A ) Mice of indicated genotypes were administered TM (0.17 mg/g) to induce recombination, and sacrificed 9 months later. ( B - E ) H&E staining of pancreata from mice of indicated genotypes. ( F - M ) IHC for the duct markers CK19 and SOX9, indicating upregulation in both acinar-to-ductal metaplasia (ADM) and PanINs. ( N - Q ) IHC for the PanIN marker, CLDN18, highlighting intermittent PanIN formation in Kras G12D mice and widespread lesion development in Ptf1a conditional knock-out (cKO); Kras G12D . ( R - U ) Alcian Blue staining, indicating PanIN lesions in Kras G12D and Ptf1a cKO ; Kras G12D pancreata. ( V - Y ) Sirius Red staining, highlighting local and widespread fibrosis in Kras G12D and Ptf1a cKO; Kras G12D mice, respectively. Scale bars: ( B - E ) 200 um; ( F - I ) 200 um; ( J - Q ) 25 um; ( R - U ) 500 um; ( V - Y ) 200 um. DOI: http://dx.doi.org/ Figure 2--figure supplement 1. Schematic of mouse alleles used in this study. Schematic representations of the alleles present in the genotypes referred to, in shorthand, as control, Ptf1a cKO, Kras G12D and Ptf1a cKO; Kras G12D (see Table 1 for additional details). DOI: http://dx.doi.org/ Figure 2--figure supplement 2. Ptf1a CreERT deletion efficiency following tamoxifen treatment. 6-8-week-old mice of indicated genotypes were administered according to low- or high-dose regimens (1 x 0.17 mg/g or 0.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6--figure supplement 1. Mucinous metaplasia associated with hyperactive MEK-ERK signaling in caerulein-treated Ptf1a cKO pancreata. ( A , B ) IHC for the PanIN markers Claudin-18 ( A ) and MUC5ac ( B ) in caerulein-treated Ptf1a cKO with corresponding positive and negative controls. Green arrows indicate weakly Claudin-18 positive or Muc5ac positive lesions and red arrows indicate Claudin-18/Muc5ac negative metaplasia. ( C - F ) IHC for phosphorylated-ERK (p-ERK) on control and Ptf1a cKO pancreata 1 week following caerulein treatment. Enlarged boxed area highlights mucinous metaplasia-like lesions from caerulein-treated Ptf1a cKO pancreata with strong nuclear p-ERK signal. Scale bars: 200 mum. DOI: http://dx.doi.org/

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CLDN18.1 regulates IGF-1R signaling ( a ) IPA analysis of 135 genes differentially expressed in AT2 cells freshly isolated from Cldn18 -/- and WT mice (FC > 2, p < 0.05). Line graph represents the ratio between the number of genes in a given pathway that are present in the list of differentially regulated genes divided by the total number of genes that make up that pathway in the reference gene set. The significance values for the canonical pathways represented by bars were calculated using right-tailed Fisher's exact test. ( b ) Western analysis and quantitation of IGF-1R and AKT phosphorylation in E18 lung of wild type and Cldn18 -/- mouse mice. ( c ) The top 77 proteins affected by Dox in H23/C18 cells were interrogated using IPA as in Panel ( a ). ( d ) Representative Western blots and quantitative analysis of IGF-1R and AKT phosphorylation in Dox-treated versus control H23/C18 cells. In ( b,d ), * indicates p < 0.05, n = 3, Z -test. Western blot data shown in figures d , b and S7 a of the manuscript are from the same experiment.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Relative expression of claudins 3 and 18 in wild-type, cryptorchid, and orchiopexied dog testes. ( A ) Vimentin and claudin-3. ( B ) Vimentin and claudin-18 were detected in wild-type, cryptorchid, and orchiopexied dog testes by immunostaining analysis. Vimentin, claudin-3, and claudin-18 were localized in the cell membrane near the basement membrane of the testes. Scale bars = 50 mum; n = 3, two pairs of testes.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Ductal deletion of Hnf1b leads to PanIN by 2 months. ( A ) H&E staining of mutant pancreata showing epithelial structures composed of columnar cells with abundant supranuclear cytoplasm and basally located nuclei. ( B ) Alcian blue staining. Columnar mutant epithelial cells showed blue-stained supranuclear mucin. ( C ) PanIN marker claudin 18 (brown) immunohistochemistry. ( D ) Sox9 (red) and GFP (green) immunostaining. Sox9+ ADM structures did not derive from Hnf1b-targeted GFP+ cells. ( E ) Quantification of the relative surface of acini, adipocytes, fibrosis/infiltrates, ADM, and PanINs in mutants (n = 6). Scale bars : 100 mum. Control, n >= 3; mutant, n >= 3 for histology and immunohistochemistry. DAPI, 4',6-diamidino-2-phenylindole.

700178

Antibody data