- 38-8000 - Invitrogen Antibodies CLDN18 antibody

Submitted references The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms.

Balbinot C, Armant O, Elarouci N, Marisa L, Martin E, De Clara E, Onea A, Deschamps J, Beck F, Freund JN, Duluc I
The Journal of experimental medicine 2018 Mar 5;215(3):911-926

Frequent CLDN18-ARHGAP fusion in highly metastatic diffuse-type gastric cancer with relatively early onset.

Tanaka A, Ishikawa S, Ushiku T, Yamazawa S, Katoh H, Hayashi A, Kunita A, Fukayama M
Oncotarget 2018 Jun 29;9(50):29336-29350

Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke.

Wolf L, Herr C, Niederstraßer J, Beisswenger C, Bals R
PloS one 2017;12(7):e0180092

P2X7R-dependent regulation of glycogen synthase kinase 3β and claudin-18 in alveolar epithelial type I cells of mice lung.

Barth K, Bläsche R, Neißer A, Bramke S, Frank JA, Kasper M
Histochemistry and cell biology 2016 Dec;146(6):757-768

Expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma: association with features of differentiation.

Soini Y, Takasawa A, Eskelinen M, Juvonen P, Kärjä V, Hasegawa T, Murata M, Tanaka S, Kojima T, Sawada N
Journal of clinical pathology 2012 May;65(5):431-6

Claudin 18 is a novel negative regulator of bone resorption and osteoclast differentiation.

Linares GR, Brommage R, Powell DR, Xing W, Chen ST, Alshbool FZ, Lau KH, Wergedal JE, Mohan S
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Jul;27(7):1553-65

Claudins 10 and 18 are predominantly expressed in lung adenocarcinomas and in tumors of nonsmokers.

Merikallio H, Pääkkö P, Harju T, Soini Y
International journal of clinical and experimental pathology 2011;4(7):667-73

Claudin-18 is an early-stage marker of pancreatic carcinogenesis.

Tanaka M, Shibahara J, Fukushima N, Shinozaki A, Umeda M, Ishikawa S, Kokudo N, Fukayama M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 Oct;59(10):942-52

S1P3 receptor-induced reorganization of epithelial tight junctions compromises lung barrier integrity and is potentiated by TNF.

Gon Y, Wood MR, Kiosses WB, Jo E, Sanna MG, Chun J, Rosen H
Proceedings of the National Academy of Sciences of the United States of America 2005 Jun 28;102(26):9270-5

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of mouse lung homogenates using Claudin-18 (C-Term) Polyclonal Antibody, Rabbit (C-term) (Product # 38-8000).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin-18 (C-Term) showing staining in the membrane of paraffin-embedded human lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-18 (C-Term) Rabbit Polyclonal Antibody (Product # 38-8000) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin-18 (C-Term) showing staining in the membrane of paraffin-embedded human stomach tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-18 (C-Term) Rabbit Polyclonal Antibody (Product # 38-8000) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin-18 (C-Term) showing staining in the membrane of paraffin-embedded Mouse lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-18 (C-Term) Rabbit Polyclonal Antibody (Product # 38-8000) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 3 RAGE promotes the differentiation and formation of an intact barrier function in primary murine alveolar epithelial cells. Alveolar epithelial cells were isolated from WT and RAGE -/- mice and analyzed in an air-liquid interface culture system. (A) TEER of isolated alveolar epithelial cells was measured for a period of five days; n = 12 per group. Relative mRNA induction of alveolar epithelial type 2 cell marker surfactant protein C (B), alveolar epithelial type 1 cell marker aquaporin-5 (AQP5) (C) and the tight junction proteins claudin 18 (D), ZO-1 (E) and occludin (F) was determined by qRT-PCR; n = 6 per group. The proteins of cell lysate of alveolar epithelial cells at day 2 and 4 post isolation were separated by SDS-PAGE and stained with antibodies against alpha-Tubulin (alphaTub), aquaporin-5 (AQP5), pro-surfactant protein C (Sp-C), and claudin 18 (Cldn18) (G). The bands of the blot were densitometrically analyzed and the results are shown for AQP5 (H), SP-C (I), and Cldn18 (J). Data are shown as mean +- SEM. *p < 0.05; **p < 0.01 and ***p < 0.001.

38-8000

Antibody data