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- Antibody Data
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- RQ8305 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- METAP2 Antibody / Methionine aminopeptidase 2
- Antibody type
- Polyclonal
- Description
- This highly specific METAP2 antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the METAP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat pancreas, 2) rat testis, 3) rat brain, 4) rat PC-12, 5) mouse pancreas, 6) mouse testis, 7) mouse brain and 8) mouse L929 cell lysate with METAP2 antibody. Predicted molecular weight: 50-53 kDa but may be observed at higher molecular weights due to glycosylation.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human MCF7, 3) human HeLa, 4) human Jurkat, 5) human DLD-1, 6) human HT1080, 7) human HL60 and 8) monkey COS-7 cell lysate with METAP2 antibody. Predicted molecular weight: 50-53 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil tissue with METAP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human brain tissue with METAP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colorectal adenocarcinoma tissue with METAP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
