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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- GTX14372 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX14372, RRID:AB_367071
- Product name
- Ubiquitin antibody
- Antibody type
- Polyclonal
- Antigen
- This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde. Whole Human ubiquitin protein was used as the immunogen.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 250µg
- Concentration
- 5 mg/ml
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX14372was tested by immunoblot against E.coli lysates expressing a Ubiquitin GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity o
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Ubiquitin. Anti-Ubiquitin antibody (GTX14372), generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by western blot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche).
- Validation comment
- WB
