M02848-3

antibody from Boster Biological Technology

Targeting: UBB FLJ25987, MGC8385

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M02848-3

Provider

Boster Biological Technology

Product name

Anti-Ubiquitin UBB Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Ubiquitin/UBB detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat Ubiquitin/UBB information: Molecular Weight: 25762 MW; Subcellular Localization: Ubiquitin: Cytoplasm . Nucleus .

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DIG-21

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation.

Ma C, Wang X, He S, Zhang L, Bai J, Qu L, Qi J, Zheng X, Zhu X, Mei J, Guan X, Yuan H, Zhu D
Cell & bioscience 2022 Jan 28;12(1):9

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Ubr1-mediated ubiquitylation orchestrates asexual development, polar growth, and virulence-related cellular events in Beauveria bassiana.

Wang DY, Mou YN, Du X, Guan Y, Feng MG
Applied microbiology and biotechnology 2021 Apr;105(7):2747-2758

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Pleiotropic effects of Ubi4, a polyubiquitin precursor required for ubiquitin accumulation, conidiation and pathogenicity of a fungal insect pathogen.

Wang DY, Ren K, Tong SM, Ying SH, Feng MG
Environmental microbiology 2020 Jul;22(7):2564-2580

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using Ubiquitin Antibody(M02848-3)UBB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBB Antibody (M02848-3)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image