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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ABIN568040 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ubiquitin (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Ubiquitin
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mL
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western Blot. 5 ?g of recombinant protein from Phytophora sp. (Lane 1), human (Lane 2), Arabidopsis thaliana His-tagged ubiquitin (Lane 3), Arabidopsis thaliana His-tagged SUMO protein (Lane 4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1/10000 anti-Ubiquitin antibody (1h), followed by incubation with 1/15000 secondary anti-Rabbit antibodies (1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.
