V3933

antibody from NSJ Bioreagents

Targeting: UBB FLJ25987, MGC8385

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3933

Provider

NSJ Bioreagents

Product name

Ubiquitin Antibody

Antibody type

Monoclonal

Description

This highly specific Ubiquitin antibody is suitable for use in Flow cytometry/Western blot/Immunofluorescence/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

UBB/1748

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the Ubiquitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Raji cell lysate with Ubiquitin antibody (clone UBB/1748). Expected molecular weight ~9 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Raji cell lysate with Ubiquitin antibody (clone UBB/1748). Expected molecular weight ~9 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HeLa cell lysate with Ubiquitin antibody (clone UBB/1748). Expected molecular weight ~9 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Ubiquitin antibody (clone UBB/1748). These results demonstrate the foremost specificity of the UBB/1748 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Ubiquitin antibody (clone UBB/1748) as confirmation of integrity and purity.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Ubiquitin antibody (clone UBB/1748) as confirmation of integrity and purity.