Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (STJ11100145) at 1:1000 dilution. NIH/3T3 cells were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using Ubiquitin rabbit polyclonal antibody (STJ11100145) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Ubiquitin rabbit polyclonal antibody (STJ11100145) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Ubiquitin rabbit polyclonal antibody (STJ11100145) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.