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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- R1199 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1199, RRID:AB_1008340
- Product name
- anti Ubiquitin
- Antibody type
- Polyclonal
- Antigen
- This purified antibody was prepared from Rabbit serum after repeated immunizations with Ubiquitin coupled to Rabbit IgG using glutaraldehyde.
- Reactivity
- Yeast
- Host
- Rabbit
- Vial size
- 0.5 mg
- Concentration
- 5.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Figure 1: Immunoblot of Ubiquitin. Anti-Ubiquitin antibody, generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by Immunoblot against total cell extract from yeast. Dilution of the antibody between 1/200 and 1/1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1/500 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1/2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at RT. A Chemi-luminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
