Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP20051SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP20051SU-N, RRID:AB_10669880
- Product name
- anti Ubiquitin (N-term)
- Antibody type
- Polyclonal
- Antigen
- Recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 ml
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot. 5 μg of recombinant protein from Phytophora sp. (Lane 1), human (Lane 2), Arabidopsis thaliana His-tagged ubiquitin (Lane 3), Arabidopsis thaliana His-tagged SUMO protein (Lane 4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1/10000 anti-Ubiquitin antibody (1h), followed by incubation with 1/15000 secondary anti-Rabbit antibodies (1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.
