Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB703 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Ubiquitin+1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Ubiquitin+1 in Western blots. Does not detect recombinant Ubiquitin.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 82001
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Ubiquitin+1 by Western Blot. Western blot shows samples of Recombinant Human Ubiquitin+1 (Catalog # 703-UB) (2, 1, and 0.5 ng) and Recombinant Human Ubiquitin (Catalog # 701-UB) (2, 1, and 0.5 ng). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human Ubiquitin+1 Monoclonal Antibody (Catalog # MAB703) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Ubiquitin+1 was detected at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.