PA5-60783

antibody from Invitrogen Antibodies

Targeting: ATP5MG ATP5JG, ATP5L

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-60783

Provider

Invitrogen Antibodies

Product name

ATP5L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AQFVRNLVEK TPALVNAAVT YSKPRLATFW YYAKVELVPP TPAEIPRAIQ SLKKIVNSAQ TGSFK Highest antigen sequence identity to the following orthologs: Mouse - 78%, Rat - 72%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Comparative Proteomic Profiling Identifies Reciprocal Expression of Mitochondrial Proteins Between White and Gray Matter Lesions From Multiple Sclerosis Brains.

Rai NK, Singh V, Li L, Willard B, Tripathi A, Dutta R
Frontiers in neurology 2021;12:779003

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ATP5L in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using an ATP5L Polyclonal Antibody (Product # PA5-60783).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Cortical GML proteome profiling. (A) Volcano plot displaying the distribution of all proteins ( n = 1182) with relative protein abundance (Log2 fold change) plotted against significance level (-Log 10 P -Value). Significantly upregulated (red circle) as well as downregulated (blue circle) proteins in GMLs are also indicated. (B) Cellular identity of the significantly dysregulated GML proteins (upregulated and downregulated). (C) Venn diagram comparison of DEPs ( p < 0.05) between WMLs and GMLs. (D) IPA canonical pathways, activated (red) and inhibited (blue), associated with significantly dysregulated proteins in GMLs. (E) LFQ-based heat map of mitochondria-specific downregulated proteins in GMLs. Proteins that are common and have opposite expressions in WML proteomic data are highlighted in red. (F) PPI network analysis of downregulated proteins showing enrichment of mitochondria-related pathways. (G) MCODE analysis revealed a highly interconnected sub-network associated with oxidative phosphorylation. (H-L) PLP- stained image of (H) sub-pial lesion. IHC images showing reduced expression of NDUFA5 (I,J) and ATP5L (K,L) in demyelinated regions (red box) compared to NAGM (blue box). Scale bar- 500mum (H) and 100mum (I-L) .