Antibody data
- Antibody Data
- Antigen structure
- References [45]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [11]
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Validation data
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- Product number
- NBP2-24917 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Toll-like Receptor 8 Stability Is Regulated by Ring Finger 216 in Response to Circulating MicroRNAs.
p53-responsive TLR8 SNP enhances human innate immune response to respiratory syncytial virus.
Impaired expression and function of TLR8 in chronic HBV infection and its association with treatment responses during peg-IFN-α-2a antiviral therapy.
Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas.
Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination.
The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer.
Direct Toll-Like Receptor 8 signaling increases the functional avidity of human CD8+ T lymphocytes generated for adoptive T cell therapy strategies.
Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils.
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
Deprived TLR9 expression in apparently healthy nasal mucosa might trigger polyp-growth in chronic rhinosinusitis patients.
HIV-derived ssRNA binds to TLR8 to induce inflammation-driven macrophage foam cell formation.
Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis.
Differential partial activation phenotype and production of tumour necrosis factor-α by conventional dendritic cells in response to lipopolysaccharide in HIV+ viraemic subjects and HIV+ controllers.
Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells.
Functional polymorphisms of TLR8 are associated with hepatitis C virus infection.
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNFα in monocytes.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Neutrophils express distinct RNA receptors in a non-canonical way.
Inhibition of TLR8- and TLR4-induced Type I IFN induction by alcohol is different from its effects on inflammatory cytokine production in monocytes.
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Expression of Toll-like receptors by neonatal leukocytes.
An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis.
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
TLR activation pathways in HIV-1-exposed seronegative individuals.
Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response.
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Toll-like receptor triggering in cord blood mesenchymal stem cells.
Differentially stimulated CD4+ T cells display altered human immunodeficiency virus infection kinetics: implications for the efficacy of antiviral agents.
Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells.
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
Ciprofloxacin inhibits lipopolysaccharide-induced toll-like receptor-4 and 8 expression on human monocytes derived from adult and cord blood.
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Differentiation of primary adult microglia alters their response to TLR8-mediated activation but not their capacity as APC.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
CD molecules 2006--human cell differentiation molecules.
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells.
TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production.
TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production.
Evidence of Toll-like receptor molecules on human platelets.
Evankovich J, Lear T, Baldwin C, Chen Y, White V, Villandre J, Londino J, Liu Y, McVerry B, Kitsios GD, Mallampalli RK, Chen BB
American journal of respiratory cell and molecular biology 2020 Feb;62(2):157-167
American journal of respiratory cell and molecular biology 2020 Feb;62(2):157-167
p53-responsive TLR8 SNP enhances human innate immune response to respiratory syncytial virus.
Menendez D, Snipe J, Marzec J, Innes CL, Polack FP, Caballero MT, Schurman SH, Kleeberger SR, Resnick MA
The Journal of clinical investigation 2019 Nov 1;129(11):4875-4884
The Journal of clinical investigation 2019 Nov 1;129(11):4875-4884
Impaired expression and function of TLR8 in chronic HBV infection and its association with treatment responses during peg-IFN-α-2a antiviral therapy.
Deng G, Ge J, Liu C, Pang J, Huang Z, Peng J, Sun J, Hou J, Zhang X
Clinics and research in hepatology and gastroenterology 2017 Sep;41(4):386-398
Clinics and research in hepatology and gastroenterology 2017 Sep;41(4):386-398
Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas.
Helminen O, Huhta H, Kauppila JH, Lehenkari PP, Saarnio J, Karttunen TJ
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2017 Feb;125(2):85-92
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2017 Feb;125(2):85-92
Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination.
Jeon YK, Kim CK, Koh J, Chung DH, Ha GH
Oncotarget 2016 Jul 5;7(27):41811-41824
Oncotarget 2016 Jul 5;7(27):41811-41824
The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer.
Huhta H, Helminen O, Kauppila JH, Salo T, Porvari K, Saarnio J, Lehenkari PP, Karttunen TJ
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2016 Aug;64(8):470-82
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2016 Aug;64(8):470-82
Direct Toll-Like Receptor 8 signaling increases the functional avidity of human CD8+ T lymphocytes generated for adoptive T cell therapy strategies.
Chatillon JF, Hamieh M, Bayeux F, Abasq C, Fauquembergue E, Drouet A, Guisier F, Latouche JB, Musette P
Immunity, inflammation and disease 2015 Mar;3(1):1-13
Immunity, inflammation and disease 2015 Mar;3(1):1-13
Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils.
Zimmermann M, Aguilera FB, Castellucci M, Rossato M, Costa S, Lunardi C, Ostuni R, Girolomoni G, Natoli G, Bazzoni F, Tamassia N, Cassatella MA
Nature communications 2015 Jan 23;6:6061
Nature communications 2015 Jan 23;6:6061
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
Jamin A, Dehoux L, Dossier C, Fila M, Heming N, Monteiro RC, Deschênes G
Clinical and experimental immunology 2015 Dec;182(3):332-45
Clinical and experimental immunology 2015 Dec;182(3):332-45
Deprived TLR9 expression in apparently healthy nasal mucosa might trigger polyp-growth in chronic rhinosinusitis patients.
Tengroth L, Arebro J, Kumlien Georén S, Winqvist O, Cardell LO
PloS one 2014;9(8):e105618
PloS one 2014;9(8):e105618
HIV-derived ssRNA binds to TLR8 to induce inflammation-driven macrophage foam cell formation.
Bernard MA, Han X, Inderbitzin S, Agbim I, Zhao H, Koziel H, Tachado SD
PloS one 2014;9(8):e104039
PloS one 2014;9(8):e104039
Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis.
Youn JC, Shim HS, Lee JS, Ji AY, Oh J, Hong N, Lee HS, Park S, Lee SH, Choi D, Chung N, Kang SM
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2014 May-Jun;23(3):139-44
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2014 May-Jun;23(3):139-44
Differential partial activation phenotype and production of tumour necrosis factor-α by conventional dendritic cells in response to lipopolysaccharide in HIV+ viraemic subjects and HIV+ controllers.
Camacho-Sandoval R, Del Río Estrada PM, Rivero-Arrieta A, Reyes-Terán G, Bonifaz LC
Clinical and experimental immunology 2014 Dec;178(3):489-503
Clinical and experimental immunology 2014 Dec;178(3):489-503
Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells.
Green TL, Santos MF, Ejaeidi AA, Craft BS, Lewis RE, Cruse JM
Experimental and molecular pathology 2014 Aug;97(1):44-8
Experimental and molecular pathology 2014 Aug;97(1):44-8
Functional polymorphisms of TLR8 are associated with hepatitis C virus infection.
Wang CH, Eng HL, Lin KH, Liu HC, Chang CH, Lin TM
Immunology 2014 Apr;141(4):540-8
Immunology 2014 Apr;141(4):540-8
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Abdi J, Mutis T, Garssen J, Redegeld F
PloS one 2013;8(4):e60671
PloS one 2013;8(4):e60671
Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNFα in monocytes.
Chamberlain ND, Kim SJ, Vila OM, Volin MV, Volkov S, Pope RM, Arami S, Mandelin AM 2nd, Shahrara S
Annals of the rheumatic diseases 2013 Mar;72(3):418-26
Annals of the rheumatic diseases 2013 Mar;72(3):418-26
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Neutrophils express distinct RNA receptors in a non-canonical way.
Berger M, Hsieh CY, Bakele M, Marcos V, Rieber N, Kormann M, Mays L, Hofer L, Neth O, Vitkov L, Krautgartner WD, von Schweinitz D, Kappler R, Hector A, Weber A, Hartl D
The Journal of biological chemistry 2012 Jun 1;287(23):19409-17
The Journal of biological chemistry 2012 Jun 1;287(23):19409-17
Inhibition of TLR8- and TLR4-induced Type I IFN induction by alcohol is different from its effects on inflammatory cytokine production in monocytes.
Pang M, Bala S, Kodys K, Catalano D, Szabo G
BMC immunology 2011 Sep 30;12:55
BMC immunology 2011 Sep 30;12:55
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Dasgupta G, Chentoufi AA, You S, Falatoonzadeh P, Urbano LA, Akhtarmalik A, Nguyen K, Ablabutyan L, Nesburn AB, BenMohamed L
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Expression of Toll-like receptors by neonatal leukocytes.
Dasari P, Zola H, Nicholson IC
Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 2011 Mar;22(2):221-8
Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 2011 Mar;22(2):221-8
An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis.
Schwab N, Zozulya AL, Kieseier BC, Toyka KV, Wiendl H
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5368-74
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5368-74
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
Wu J, Meng Z, Jiang M, Zhang E, Trippler M, Broering R, Bucchi A, Krux F, Dittmer U, Yang D, Roggendorf M, Gerken G, Lu M, Schlaak JF
Immunology 2010 Mar;129(3):363-74
Immunology 2010 Mar;129(3):363-74
TLR activation pathways in HIV-1-exposed seronegative individuals.
Biasin M, Piacentini L, Lo Caputo S, Naddeo V, Pierotti P, Borelli M, Trabattoni D, Mazzotta F, Shearer GM, Clerici M
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2710-7
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2710-7
Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response.
Panda A, Qian F, Mohanty S, van Duin D, Newman FK, Zhang L, Chen S, Towle V, Belshe RB, Fikrig E, Allore HG, Montgomery RR, Shaw AC
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2518-27
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2518-27
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
Clancy RM, Alvarez D, Komissarova E, Barrat FJ, Swartz J, Buyon JP
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg HH, Wesch D, Kabelitz D
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Toll-like receptor triggering in cord blood mesenchymal stem cells.
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG, Netea MG, Latuhihin T, Bergevoet S, Raymakers RA, Kögler G, Figdor CC, Adema GJ, Torensma R
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Differentially stimulated CD4+ T cells display altered human immunodeficiency virus infection kinetics: implications for the efficacy of antiviral agents.
Vatakis DN, Nixon CC, Bristol G, Zack JA
Journal of virology 2009 Apr;83(7):3374-8
Journal of virology 2009 Apr;83(7):3374-8
Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells.
Clark RA, Huang SJ, Murphy GF, Mollet IG, Hijnen D, Muthukuru M, Schanbacher CF, Edwards V, Miller DM, Kim JE, Lambert J, Kupper TS
The Journal of experimental medicine 2008 Sep 29;205(10):2221-34
The Journal of experimental medicine 2008 Sep 29;205(10):2221-34
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
Cohen PA, Koski GK, Czerniecki BJ, Bunting KD, Fu XY, Wang Z, Zhang WJ, Carter CS, Awad M, Distel CA, Nagem H, Paustian CC, Johnson TD, Tisdale JF, Shu S
Blood 2008 Sep 1;112(5):1832-43
Blood 2008 Sep 1;112(5):1832-43
Ciprofloxacin inhibits lipopolysaccharide-induced toll-like receptor-4 and 8 expression on human monocytes derived from adult and cord blood.
Kaji M, Tanaka J, Sugita J, Kato N, Ibata M, Shono Y, Ohta S, Kondo T, Asaka M, Imamura M
Annals of hematology 2008 Mar;87(3):229-31
Annals of hematology 2008 Mar;87(3):229-31
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
Sacre SM, Lo A, Gregory B, Simmonds RE, Williams L, Feldmann M, Brennan FM, Foxwell BM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
Sacre SM, Lo A, Gregory B, Simmonds RE, Williams L, Feldmann M, Brennan FM, Foxwell BM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Murciano C, Villamón E, Yáñez A, Murciano J, Mir A, O'Connor JE, Gozalbo D, Gil ML
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
Differentiation of primary adult microglia alters their response to TLR8-mediated activation but not their capacity as APC.
Zuiderwijk-Sick EA, van der Putten C, Bsibsi M, Deuzing IP, de Boer W, Persoon-Deen C, Kondova I, Boven LA, van Noort JM, 't Hart BA, Amor S, Bajramovic JJ
Glia 2007 Nov 15;55(15):1589-600
Glia 2007 Nov 15;55(15):1589-600
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
CD molecules 2006--human cell differentiation molecules.
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Bühring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
Lee J, Wu CC, Lee KJ, Chuang TH, Katakura K, Liu YT, Chan M, Tawatao R, Chung M, Shen C, Cottam HB, Lai MM, Raz E, Carson DA
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Xu S, Koldovsky U, Xu M, Wang D, Fitzpatrick E, Son G, Koski G, Czerniecki BJ
Surgery 2006 Aug;140(2):170-8
Surgery 2006 Aug;140(2):170-8
Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells.
Levy O, Suter EE, Miller RL, Wessels MR
Blood 2006 Aug 15;108(4):1284-90
Blood 2006 Aug 15;108(4):1284-90
TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production.
Hart OM, Athie-Morales V, O'Connor GM, Gardiner CM
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1636-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1636-42
TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production.
Hart OM, Athie-Morales V, O'Connor GM, Gardiner CM
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1636-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Aug 1;175(3):1636-42
Evidence of Toll-like receptor molecules on human platelets.
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O
Immunology and cell biology 2005 Apr;83(2):196-8
Immunology and cell biology 2005 Apr;83(2):196-8
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - Analysis of TLR8 in human Ramos cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - All the cell lines expressed mRNA for TLR8 (Figure 1) and displayed a strong expression of TLR8 protein (Figure 2). Lysates of human intestinal tissue and mouse splenocytes were used as positive controls. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY licence.
Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml.
Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular flow analysis of 10^6 human lymphocytes using 1 ug of TLR8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR8 antibody. Goat anti-mouse IgG1 PE conjugate (BD) was used as secondary.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - An intracellular stain was performed on A549 cells with TLR8 (44C143) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at RT, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems] ( A). A negative control, HEK293 cells, was also stained to ensure antibody specificity (B).
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of CD14-/CD11c+/TLR8+ myeloid dendritic cells (mDCs). Cells were stained for surface markers CD14 and CD11c, and intracellular stained for TLR8. CD14- cells were gated and stained with FITC-conjugated TLR8 (NBP2-24917), and APC-conjugated CD11c (right).
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in 10^6 human PBMCs using 0.5 ug of NBP2-24817, 0.25 ug of anti-human CD14, and 0.5 ug of isotype control. Novus's TLR intracellular staining flow assay kit was used to test this product.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of TLR8 in 10^6 Ramos cells using 0.5 ugs of this antibody. The shaded histogram represents Ramos cells alone, green represents isotype control, and red represents TLR8 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of TLR8 in 10^6 human lymphocytes using 1 ug of this antibody. The shaded histogram represents lymphocytes alone, green represents isotype control, and red represents TLR8 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in Ramos cells using 2 ug of NBP2-24817. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR8 antibody.
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in BALB/c mouse splenocytes (lymphocyte gate) using 2 ug of this antibody. Green represents isotype control ; red represents anti-TLR8 antibody.
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- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular expression of TLR8 in monocyte-derived macrophages (MDMs). MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. Intracellular expression was determined by flow cytometry. Representative profiles were similar in four independent experiments (n=4 subjects). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY licence.
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- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - HEK cells expressing TLR8 were treated with ssRNA40-Alexa 546 followed by fixation, permeablization and staining for TLR8 using anti-TLR8 Alexa 488 conjugate or isotype control IgG1/Kappa Alexa 488. TLR8 detection is shown along the horizontal axis, and ssRNA40 Alexa 546 detection is shown along the vertical axis. Direct excitation (488 and 561 nm lasers) of both fluorophores (Alexa 488 and Alexa 546) allowed quantitation of cells staining positive for TLR8 (anti-TLR8-Alexa488) and cells that had taken up ssRNA40-Alexa 546. Single-stranded RNA40 binds TLR8 (flow cytometry FRET assay). A representative experiment is shown from three independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY licence.
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- Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Expression of TLR8 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR8 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR8. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY licence.