Antibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Flow cytometry [4]
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Validation data
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- Product number
- NBP2-24817 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rabbit
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Impaired expression and function of TLR8 in chronic HBV infection and its association with treatment responses during peg-IFN-α-2a antiviral therapy.
Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils.
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
Differential partial activation phenotype and production of tumour necrosis factor-α by conventional dendritic cells in response to lipopolysaccharide in HIV+ viraemic subjects and HIV+ controllers.
Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells.
Functional polymorphisms of TLR8 are associated with hepatitis C virus infection.
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNFα in monocytes.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Neutrophils express distinct RNA receptors in a non-canonical way.
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis.
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
TLR activation pathways in HIV-1-exposed seronegative individuals.
Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response.
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Toll-like receptor triggering in cord blood mesenchymal stem cells.
Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells.
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
Ciprofloxacin inhibits lipopolysaccharide-induced toll-like receptor-4 and 8 expression on human monocytes derived from adult and cord blood.
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Differentiation of primary adult microglia alters their response to TLR8-mediated activation but not their capacity as APC.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
CD molecules 2006--human cell differentiation molecules.
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells.
Deng G, Ge J, Liu C, Pang J, Huang Z, Peng J, Sun J, Hou J, Zhang X
Clinics and research in hepatology and gastroenterology 2017 Sep;41(4):386-398
Clinics and research in hepatology and gastroenterology 2017 Sep;41(4):386-398
Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils.
Zimmermann M, Aguilera FB, Castellucci M, Rossato M, Costa S, Lunardi C, Ostuni R, Girolomoni G, Natoli G, Bazzoni F, Tamassia N, Cassatella MA
Nature communications 2015 Jan 23;6:6061
Nature communications 2015 Jan 23;6:6061
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
Jamin A, Dehoux L, Dossier C, Fila M, Heming N, Monteiro RC, Deschênes G
Clinical and experimental immunology 2015 Dec;182(3):332-45
Clinical and experimental immunology 2015 Dec;182(3):332-45
Differential partial activation phenotype and production of tumour necrosis factor-α by conventional dendritic cells in response to lipopolysaccharide in HIV+ viraemic subjects and HIV+ controllers.
Camacho-Sandoval R, Del Río Estrada PM, Rivero-Arrieta A, Reyes-Terán G, Bonifaz LC
Clinical and experimental immunology 2014 Dec;178(3):489-503
Clinical and experimental immunology 2014 Dec;178(3):489-503
Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells.
Green TL, Santos MF, Ejaeidi AA, Craft BS, Lewis RE, Cruse JM
Experimental and molecular pathology 2014 Aug;97(1):44-8
Experimental and molecular pathology 2014 Aug;97(1):44-8
Functional polymorphisms of TLR8 are associated with hepatitis C virus infection.
Wang CH, Eng HL, Lin KH, Liu HC, Chang CH, Lin TM
Immunology 2014 Apr;141(4):540-8
Immunology 2014 Apr;141(4):540-8
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Abdi J, Mutis T, Garssen J, Redegeld F
PloS one 2013;8(4):e60671
PloS one 2013;8(4):e60671
Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNFα in monocytes.
Chamberlain ND, Kim SJ, Vila OM, Volin MV, Volkov S, Pope RM, Arami S, Mandelin AM 2nd, Shahrara S
Annals of the rheumatic diseases 2013 Mar;72(3):418-26
Annals of the rheumatic diseases 2013 Mar;72(3):418-26
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Neutrophils express distinct RNA receptors in a non-canonical way.
Berger M, Hsieh CY, Bakele M, Marcos V, Rieber N, Kormann M, Mays L, Hofer L, Neth O, Vitkov L, Krautgartner WD, von Schweinitz D, Kappler R, Hector A, Weber A, Hartl D
The Journal of biological chemistry 2012 Jun 1;287(23):19409-17
The Journal of biological chemistry 2012 Jun 1;287(23):19409-17
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Dasgupta G, Chentoufi AA, You S, Falatoonzadeh P, Urbano LA, Akhtarmalik A, Nguyen K, Ablabutyan L, Nesburn AB, BenMohamed L
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis.
Schwab N, Zozulya AL, Kieseier BC, Toyka KV, Wiendl H
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5368-74
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5368-74
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
Wu J, Meng Z, Jiang M, Zhang E, Trippler M, Broering R, Bucchi A, Krux F, Dittmer U, Yang D, Roggendorf M, Gerken G, Lu M, Schlaak JF
Immunology 2010 Mar;129(3):363-74
Immunology 2010 Mar;129(3):363-74
TLR activation pathways in HIV-1-exposed seronegative individuals.
Biasin M, Piacentini L, Lo Caputo S, Naddeo V, Pierotti P, Borelli M, Trabattoni D, Mazzotta F, Shearer GM, Clerici M
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2710-7
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2710-7
Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response.
Panda A, Qian F, Mohanty S, van Duin D, Newman FK, Zhang L, Chen S, Towle V, Belshe RB, Fikrig E, Allore HG, Montgomery RR, Shaw AC
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2518-27
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2518-27
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
Clancy RM, Alvarez D, Komissarova E, Barrat FJ, Swartz J, Buyon JP
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg HH, Wesch D, Kabelitz D
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Toll-like receptor triggering in cord blood mesenchymal stem cells.
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG, Netea MG, Latuhihin T, Bergevoet S, Raymakers RA, Kögler G, Figdor CC, Adema GJ, Torensma R
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells.
Clark RA, Huang SJ, Murphy GF, Mollet IG, Hijnen D, Muthukuru M, Schanbacher CF, Edwards V, Miller DM, Kim JE, Lambert J, Kupper TS
The Journal of experimental medicine 2008 Sep 29;205(10):2221-34
The Journal of experimental medicine 2008 Sep 29;205(10):2221-34
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
Cohen PA, Koski GK, Czerniecki BJ, Bunting KD, Fu XY, Wang Z, Zhang WJ, Carter CS, Awad M, Distel CA, Nagem H, Paustian CC, Johnson TD, Tisdale JF, Shu S
Blood 2008 Sep 1;112(5):1832-43
Blood 2008 Sep 1;112(5):1832-43
Ciprofloxacin inhibits lipopolysaccharide-induced toll-like receptor-4 and 8 expression on human monocytes derived from adult and cord blood.
Kaji M, Tanaka J, Sugita J, Kato N, Ibata M, Shono Y, Ohta S, Kondo T, Asaka M, Imamura M
Annals of hematology 2008 Mar;87(3):229-31
Annals of hematology 2008 Mar;87(3):229-31
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
Sacre SM, Lo A, Gregory B, Simmonds RE, Williams L, Feldmann M, Brennan FM, Foxwell BM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures.
Sacre SM, Lo A, Gregory B, Simmonds RE, Williams L, Feldmann M, Brennan FM, Foxwell BM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8002-9
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Murciano C, Villamón E, Yáñez A, Murciano J, Mir A, O'Connor JE, Gozalbo D, Gil ML
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
Differentiation of primary adult microglia alters their response to TLR8-mediated activation but not their capacity as APC.
Zuiderwijk-Sick EA, van der Putten C, Bsibsi M, Deuzing IP, de Boer W, Persoon-Deen C, Kondova I, Boven LA, van Noort JM, 't Hart BA, Amor S, Bajramovic JJ
Glia 2007 Nov 15;55(15):1589-600
Glia 2007 Nov 15;55(15):1589-600
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
CD molecules 2006--human cell differentiation molecules.
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Bühring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
Lee J, Wu CC, Lee KJ, Chuang TH, Katakura K, Liu YT, Chan M, Tawatao R, Chung M, Shen C, Cottam HB, Lai MM, Raz E, Carson DA
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Xu S, Koldovsky U, Xu M, Wang D, Fitzpatrick E, Son G, Koski G, Czerniecki BJ
Surgery 2006 Aug;140(2):170-8
Surgery 2006 Aug;140(2):170-8
Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells.
Levy O, Suter EE, Miller RL, Wessels MR
Blood 2006 Aug 15;108(4):1284-90
Blood 2006 Aug 15;108(4):1284-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [PE] [NBP2-24817] - Intracellular flow cytometric analysis of TLR8 in 10^6 human PBMCs using 0.5 ug of NBP2-24817, 0.25 ug of anti-human CD14, and 0.5 ug of isotype control. Novus's TLR intracellular staining flow assay kit was used to test this product.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [PE] [NBP2-24817] - Analysis of TLR8 in Ramos cells using 2 ug of NBP2-24817. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR8 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [PE] [NBP2-24817] - Intracellular analysis of TLR8 in BALB/c mouse splenocytes (lymphocyte gate) using 2 ug of this antibody. Green represents isotype control ; red represents anti-TLR8 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR8 Antibody (44C143) [PE] [NBP2-24817] - Intracellular expression of TLR8 in monocyte-derived macrophages (MDMs). MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. Intracellular expression was determined by flow cytometry. Representative profiles were similar in four independent experiments (n= 4 subjects). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104039) licensed under a CC-BY licence.