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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC8999T-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR8 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of TLR8 in HEK293 Human Cell Line Transfected with Human TLR8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TLR8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TLR8 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC8999T). Quadrant markers were set based on control antibody staining (Catalog # IC0041T). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
