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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-16190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR8 Monoclonal Antibody (44C143)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 44C143
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization.
van Haren SD, Dowling DJ, Foppen W, Christensen D, Andersen P, Reed SG, Hershberg RM, Baden LR, Levy O
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4413-4424
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4413-4424
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR8 in human Ramos cell lysate using a TLR8/CD288 monoclonal antibody (Product # MA5-16190) at3 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR8 in human Ramos cell lysate. Samples were incubated in TLR8 monoclonal antibody (Product # MA5-16190) followed by a goat anti-mouse Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR8 in 0.5 mg/mL Ramos (left) and Human Spleen (right) lysate. Samples were incubated in TLR8 monoclonal antibody (Product # MA5-16190). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody Image from the standard format of this antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TLR8 in formalin-fixed, paraffin-embedded human spleen. Samples were incubated in TLR8 monoclonal antibody (Product # MA5-16190) using a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR8 in 10^6 human lymphocytes using 1 µg of TLR8/CD288 monoclonal antibody (Product # MA5-16190). Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR8/CD288 monoclonal antibody (Product # MA5-16190). Goat anti-mouse IgG1 & PE conjugate was used as secondary.
