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- Antigen structure
- References [42]
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- Product number
- MHCD0300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3 Monoclonal Antibody (7D6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7D6
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references In Situ complement activation and T-cell immunity in leprosy spectrum: An immunohistological study on leprosy lesional skin.
Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs.
Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.
Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection.
Zoster Vaccination Increases the Breadth of CD4+ T Cells Responsive to Varicella Zoster Virus.
Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression.
High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Human CD3γ, but not CD3δ, haploinsufficiency differentially impairs γδ versus αβ surface TCR expression.
Expression of integrin α2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2Rγ(c) null mice.
Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells.
A novel intellectual disability syndrome caused by GPI anchor deficiency due to homozygous mutations in PIGT.
Trichosanthin-stimulated dendritic cells induce a type 2 helper T lymphocyte response through the OX40 ligand.
Systemic effector and regulatory immune responses to chlamydial antigens in trachomatous trichiasis.
NK cells as effectors of acquired immune responses: effector CD4+ T cell-dependent activation of NK cells following vaccination.
A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.
Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis.
Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses.
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.
Pre-procedural expression of Mac-1 and LFA-1 on leukocytes for prediction of late restenosis and their possible correlation with advanced coronary artery disease.
Suppression of leukemia inhibitor factor in lymphoid tissue in primary HIV infection: absence of HIV replication in gp130-positive cells.
Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
Prospective isolation of human clonogenic common myeloid progenitors.
Peripheral immature CD2-/low T cell development from type 2 to type 1 cytokine production.
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors.
Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.
Multiple co-stimulatory signals are required for triggering proliferation of T cells from human secondary lymphoid tissue.
One-round determination of seven leukocyte subsets in rhesus macaque blood by flow cytometry.
Down regulation of CD4 expression following isolation and culture of human monocytes.
Perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.
Isolation of CD34+ progenitor cells from peripheral blood by use of an automated immunomagnetic selection system: factors affecting the results.
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols.
Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.
T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.
CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.
Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro.
Growth of human hematopoietic cells in immunodeficient mice conditioned with cyclophosphamide and busulfan.
Unique CD8+ T cell-rich lymphoid aggregates in human uterine endometrium.
Bahia El Idrissi N, Iyer AM, Ramaglia V, Rosa PS, Soares CT, Baas F, Das PK
PloS one 2017;12(5):e0177815
PloS one 2017;12(5):e0177815
Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs.
Cheng L, Ma J, Li J, Li D, Li G, Li F, Zhang Q, Yu H, Yasui F, Ye C, Tsao LC, Hu Z, Su L, Zhang L
The Journal of clinical investigation 2017 Jan 3;127(1):269-279
The Journal of clinical investigation 2017 Jan 3;127(1):269-279
Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.
Wang Y, Ma CS, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, Payne K, Crestani E, Roncagalli R, Belkadi A, Kerner G, Lorenzo L, Deswarte C, Chrabieh M, Patin E, Vincent QB, Müller-Fleckenstein I, Fleckenstein B, Ailal F, Quintana-Murci L, Fraitag S, Alyanakian MA, Leruez-Ville M, Picard C, Puel A, Bustamante J, Boisson-Dupuis S, Malissen M, Malissen B, Abel L, Hovnanian A, Notarangelo LD, Jouanguy E, Tangye SG, Béziat V, Casanova JL
The Journal of experimental medicine 2016 Oct 17;213(11):2413-2435
The Journal of experimental medicine 2016 Oct 17;213(11):2413-2435
Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection.
Demers KR, Makedonas G, Buggert M, Eller MA, Ratcliffe SJ, Goonetilleke N, Li CK, Eller LA, Rono K, Maganga L, Nitayaphan S, Kibuuka H, Routy JP, Slifka MK, Haynes BF, McMichael AJ, Bernard NF, Robb ML, Betts MR
PLoS pathogens 2016 Aug;12(8):e1005805
PLoS pathogens 2016 Aug;12(8):e1005805
Zoster Vaccination Increases the Breadth of CD4+ T Cells Responsive to Varicella Zoster Virus.
Laing KJ, Russell RM, Dong L, Schmid DS, Stern M, Magaret A, Haas JG, Johnston C, Wald A, Koelle DM
The Journal of infectious diseases 2015 Oct 1;212(7):1022-31
The Journal of infectious diseases 2015 Oct 1;212(7):1022-31
Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression.
Claiborne DT, Prince JL, Scully E, Macharia G, Micci L, Lawson B, Kopycinski J, Deymier MJ, Vanderford TH, Nganou-Makamdop K, Ende Z, Brooks K, Tang J, Yu T, Lakhi S, Kilembe W, Silvestri G, Douek D, Goepfert PA, Price MA, Allen SA, Paiardini M, Altfeld M, Gilmour J, Hunter E
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 24;112(12):E1480-9
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 24;112(12):E1480-9
High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
Péguillet I, Milder M, Louis D, Vincent-Salomon A, Dorval T, Piperno-Neumann S, Scholl SM, Lantz O
Cancer research 2014 Apr 15;74(8):2204-16
Cancer research 2014 Apr 15;74(8):2204-16
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
Palin AC, Ramachandran V, Acharya S, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2682-91
Human CD3γ, but not CD3δ, haploinsufficiency differentially impairs γδ versus αβ surface TCR expression.
Muñoz-Ruiz M, Pérez-Flores V, Garcillán B, Guardo AC, Mazariegos MS, Takada H, Allende LM, Kilic SS, Sanal O, Roifman CM, López-Granados E, Recio MJ, Martínez-Naves E, Fernández-Malavé E, Regueiro JR
BMC immunology 2013 Jan 21;14:3
BMC immunology 2013 Jan 21;14:3
Expression of integrin α2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2Rγ(c) null mice.
Wong WM, Sigvardsson M, Åstrand-Grundström I, Hogge D, Larsson J, Qian H, Ekblom M
Stem cells (Dayton, Ohio) 2013 Feb;31(2):360-71
Stem cells (Dayton, Ohio) 2013 Feb;31(2):360-71
Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells.
Saghafian-Hedengren S, Sohlberg E, Theorell J, Carvalho-Queiroz C, Nagy N, Persson JO, Nilsson C, Bryceson YT, Sverremark-Ekström E
Journal of virology 2013 Dec;87(24):13446-55
Journal of virology 2013 Dec;87(24):13446-55
A novel intellectual disability syndrome caused by GPI anchor deficiency due to homozygous mutations in PIGT.
Kvarnung M, Nilsson D, Lindstrand A, Korenke GC, Chiang SC, Blennow E, Bergmann M, Stödberg T, Mäkitie O, Anderlid BM, Bryceson YT, Nordenskjöld M, Nordgren A
Journal of medical genetics 2013 Aug;50(8):521-8
Journal of medical genetics 2013 Aug;50(8):521-8
Trichosanthin-stimulated dendritic cells induce a type 2 helper T lymphocyte response through the OX40 ligand.
Yan R, Zhong W, Zhu Y, Zhang X
Journal of investigational allergology & clinical immunology 2012;22(7):491-500
Journal of investigational allergology & clinical immunology 2012;22(7):491-500
Systemic effector and regulatory immune responses to chlamydial antigens in trachomatous trichiasis.
Gall A, Horowitz A, Joof H, Natividad A, Tetteh K, Riley E, Bailey RL, Mabey DC, Holland MJ
Frontiers in microbiology 2011;2:10
Frontiers in microbiology 2011;2:10
NK cells as effectors of acquired immune responses: effector CD4+ T cell-dependent activation of NK cells following vaccination.
Horowitz A, Behrens RH, Okell L, Fooks AR, Riley EM
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 1;185(5):2808-18
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 1;185(5):2808-18
A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.
Grahmann PR, Braun RK
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 2008 Jun;12(6):636-44
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 2008 Jun;12(6):636-44
Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis.
Lünemann JD, Frey O, Eidner T, Baier M, Roberts S, Sashihara J, Volkmer R, Cohen JI, Hein G, Kamradt T, Münz C
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):991-1000
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):991-1000
Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses.
Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C
Blood 2008 Aug 15;112(4):1231-9
Blood 2008 Aug 15;112(4):1231-9
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
Contamin H, Loizon S, Bourreau E, Michel JC, Garraud O, Mercereau-Puijalon O, Behr C
Journal of immunological methods 2005 Feb;297(1-2):61-71
Journal of immunological methods 2005 Feb;297(1-2):61-71
Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.
Canonico B, Zamai L, Burattini S, Granger V, Mannello F, Gobbi P, Felici C, Falcieri E, Reilly JT, Barnett D, Papa S
Journal of immunological methods 2004 Dec;295(1-2):67-78
Journal of immunological methods 2004 Dec;295(1-2):67-78
Pre-procedural expression of Mac-1 and LFA-1 on leukocytes for prediction of late restenosis and their possible correlation with advanced coronary artery disease.
Rahimi K, Maerz HK, Zotz RJ, Tárnok A
Cytometry. Part B, Clinical cytometry 2003 May;53(1):63-9
Cytometry. Part B, Clinical cytometry 2003 May;53(1):63-9
Suppression of leukemia inhibitor factor in lymphoid tissue in primary HIV infection: absence of HIV replication in gp130-positive cells.
Tjernlund A, Fleener Z, Behbahani H, Connick E, Sönnerborg A, Broström C, Goh LE, Spetz AL, Patterson BK, Andersson J
AIDS (London, England) 2003 Jun 13;17(9):1303-10
AIDS (London, England) 2003 Jun 13;17(9):1303-10
Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.
Braun RK, Foerster M, Grahmann PR, Haefner D, Workalemahu G, Kroegel C
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):19-27
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):19-27
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
Stacchini A, Demurtas A, Godio L, Martini G, Antinoro V, Palestro G
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):10-8
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):10-8
Prospective isolation of human clonogenic common myeloid progenitors.
Manz MG, Miyamoto T, Akashi K, Weissman IL
Proceedings of the National Academy of Sciences of the United States of America 2002 Sep 3;99(18):11872-7
Proceedings of the National Academy of Sciences of the United States of America 2002 Sep 3;99(18):11872-7
Peripheral immature CD2-/low T cell development from type 2 to type 1 cytokine production.
Loza MJ, Perussia B
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3061-8
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3061-8
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
Telford WG, Moss MW, Morseman JP, Allnutt FC
Cytometry 2001 May 1;44(1):16-23
Cytometry 2001 May 1;44(1):16-23
Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors.
McIlroy D, Troadec C, Grassi F, Samri A, Barrou B, Autran B, Debré P, Feuillard J, Hosmalin A
Blood 2001 Jun 1;97(11):3470-7
Blood 2001 Jun 1;97(11):3470-7
Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.
Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6899-906
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6899-906
Multiple co-stimulatory signals are required for triggering proliferation of T cells from human secondary lymphoid tissue.
Agrawal SG, Marquet J, Plumas J, Rouard H, Delfau-Larue MH, Gaulard P, Boumsell L, Reyes F, Bensussan A, Farcet JP
International immunology 2001 Apr;13(4):441-50
International immunology 2001 Apr;13(4):441-50
One-round determination of seven leukocyte subsets in rhesus macaque blood by flow cytometry.
Lafont BA, Gloeckler L, D'Hautcourt JL, Gut JP, Aubertin AM
Cytometry 2000 Nov 1;41(3):193-202
Cytometry 2000 Nov 1;41(3):193-202
Down regulation of CD4 expression following isolation and culture of human monocytes.
Graziani-Bowering GM, Filion LG
Clinical and diagnostic laboratory immunology 2000 Mar;7(2):182-91
Clinical and diagnostic laboratory immunology 2000 Mar;7(2):182-91
Perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.
Gopinath R, Hanna LE, Kumaraswami V, Perumal V, Kavitha V, Vijayasekaran V, Nutman TB
Infection and immunity 2000 Jan;68(1):93-9
Infection and immunity 2000 Jan;68(1):93-9
Isolation of CD34+ progenitor cells from peripheral blood by use of an automated immunomagnetic selection system: factors affecting the results.
Martín-Henao GA, Picón M, Amill B, Querol S, González JR, Martínez C, Martino R, Ferrá C, Brunet S, Grañena A, Sierra J, García J
Transfusion 2000 Jan;40(1):35-43
Transfusion 2000 Jan;40(1):35-43
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
Le Cleach L, Delaire S, Boumsell L, Bagot M, Bourgault-Villada I, Bensussan A, Roujeau JC
Clinical and experimental immunology 2000 Jan;119(1):225-30
Clinical and experimental immunology 2000 Jan;119(1):225-30
Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols.
Kappelmayer J, Gratama JW, Karászi E, Menéndez P, Ciudad J, Rivas R, Orfao A
Journal of immunological methods 2000 Aug 28;242(1-2):53-65
Journal of immunological methods 2000 Aug 28;242(1-2):53-65
Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.
Paz-Miguel JE, Flores R, Sánchez-Velasco P, Ocejo-Vinyals G, Escribano de Diego J, López de Rego J, Leyva-Cobián F
Journal of immunology (Baltimore, Md. : 1950) 1999 Nov 15;163(10):5399-410
Journal of immunology (Baltimore, Md. : 1950) 1999 Nov 15;163(10):5399-410
T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.
Cron RQ, Bort SJ, Wang Y, Brunvand MW, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 1999 Jan 15;162(2):860-70
Journal of immunology (Baltimore, Md. : 1950) 1999 Jan 15;162(2):860-70
CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.
Kim CH, Pelus LM, White JR, Applebaum E, Johanson K, Broxmeyer HE
Journal of immunology (Baltimore, Md. : 1950) 1998 Mar 1;160(5):2418-24
Journal of immunology (Baltimore, Md. : 1950) 1998 Mar 1;160(5):2418-24
Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro.
Verhasselt B, De Smedt M, Verhelst R, Naessens E, Plum J
Blood 1998 Jan 15;91(2):431-40
Blood 1998 Jan 15;91(2):431-40
Growth of human hematopoietic cells in immunodeficient mice conditioned with cyclophosphamide and busulfan.
Basch RS, Quito FL, Beh J, Hirst JA
Stem cells (Dayton, Ohio) 1997;15(4):314-23
Stem cells (Dayton, Ohio) 1997;15(4):314-23
Unique CD8+ T cell-rich lymphoid aggregates in human uterine endometrium.
Yeaman GR, Guyre PM, Fanger MW, Collins JE, White HD, Rathbun W, Orndorff KA, Gonzalez J, Stern JE, Wira CR
Journal of leukocyte biology 1997 Apr;61(4):427-35
Journal of leukocyte biology 1997 Apr;61(4):427-35
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD3 was performed in Jurkat cells. Cells were fixed with 1% paraformaldehyde for 20 mins and blocked in PBS containing 2.5% BSA for 45 minutes at room temperature. Cells were labeled with CD3 Mouse Monoclonal Antibody (MHCD03004, red histogram) at a dilution of 1:20 or a matched isotype control (pink histogram) in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. A representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD3 was performed in human peripheral blood cells. Cells were blocked in PBS containing 2.5% BSA for 45 minutes and were labeled with CD3 Mouse Monoclonal Antibody (Product # MHCD0300-4) at a dilution of 1:20 in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 minutes at room temperature. Cells were acquired using an Attune® Acoustic Focusing Cytometer and cells in the lymphocyte gate were used for analysis. Red histogram represents CD3 positive cells, pink histogram represents matched isotype control and green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
