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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- MHCD0300-4 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3 Monoclonal Antibody (7D6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7D6
- Vial size
- 2 mL
- Storage
- 4° C, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD3 was performed in Jurkat cells. Cells were fixed with 1% paraformaldehyde for 20 mins and blocked in PBS containing 2.5% BSA for 45 minutes at room temperature. Cells were labeled with CD3 Mouse Monoclonal Antibody (MHCD03004, red histogram) at a dilution of 1:20 or a matched isotype control (pink histogram) in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. A representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD3 was performed in human peripheral blood cells. Cells were blocked in PBS containing 2.5% BSA for 45 minutes and were labeled with CD3 Mouse Monoclonal Antibody (Product # MHCD0300-4) at a dilution of 1:20 in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 minutes at room temperature. Cells were acquired using an Attune® Acoustic Focusing Cytometer and cells in the lymphocyte gate were used for analysis. Red histogram represents CD3 positive cells, pink histogram represents matched isotype control and green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
