MHCD0301-4

antibody from Invitrogen Antibodies

Targeting: CD247 CD3H, CD3Q, CD3Z

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MHCD0301-4

Provider

Invitrogen Antibodies

Product name

CD3 Monoclonal Antibody (7D6), FITC

Antibody type

Monoclonal

Antigen

Other

Description

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

7D6

Vial size

2 mL

Storage

4° C, store in dark

References

Submitted references

Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targeting.

Bear AS, Blanchard T, Cesare J, Ford MJ, Richman LP, Xu C, Baroja ML, McCuaig S, Costeas C, Gabunia K, Scholler J, Posey AD Jr, O'Hara MH, Smole A, Powell DJ Jr, Garcia BA, Vonderheide RH, Linette GP, Carreno BM
Nature communications 2021 Jul 16;12(1):4365

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Ligand-receptor interaction atlas within and between tumor cells and T cells in lung adenocarcinoma.

Chen Z, Yang X, Bi G, Liang J, Hu Z, Zhao M, Li M, Lu T, Zheng Y, Sui Q, Yang Y, Zhan C, Jiang W, Wang Q, Tan L
International journal of biological sciences 2020;16(12):2205-2219

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Polyreactive Broadly Neutralizing B cells Are Selected to Provide Defense against Pandemic Threat Influenza Viruses.

Guthmiller JJ, Lan LY, Fernández-Quintero ML, Han J, Utset HA, Bitar DJ, Hamel NJ, Stovicek O, Li L, Tepora M, Henry C, Neu KE, Dugan HL, Borowska MT, Chen YQ, Liu STH, Stamper CT, Zheng NY, Huang M, Palm AE, García-Sastre A, Nachbagauer R, Palese P, Coughlan L, Krammer F, Ward AB, Liedl KR, Wilson PC
Immunity 2020 Dec 15;53(6):1230-1244.e5

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Monoclonal Antibody Responses after Recombinant Hemagglutinin Vaccine versus Subunit Inactivated Influenza Virus Vaccine: a Comparative Study.

Henry C, Palm AE, Utset HA, Huang M, Ho IY, Zheng NY, Fitzgerald T, Neu KE, Chen YQ, Krammer F, Treanor JJ, Sant AJ, Topham DJ, Wilson PC
Journal of virology 2019 Nov 1;93(21)

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Identification of a human monoclonal antibody to replace equine diphtheria antitoxin for treatment of diphtheria intoxication.

Sevigny LM, Booth BJ, Rowley KJ, Leav BA, Cheslock PS, Garrity KA, Sloan SE, Thomas W Jr, Babcock GJ, Wang Y
Infection and immunity 2013 Nov;81(11):3992-4000

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Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen.

Smith K, Garman L, Wrammert J, Zheng NY, Capra JD, Ahmed R, Wilson PC
Nature protocols 2009;4(3):372-84

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Inhibition of the Bcl-xL deamidation pathway in myeloproliferative disorders.

Zhao R, Follows GA, Beer PA, Scott LM, Huntly BJ, Green AR, Alexander DR
The New England journal of medicine 2008 Dec 25;359(26):2778-89

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Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.

Asin SN, Fanger MW, Wildt-Perinic D, Ware PL, Wira CR, Howell AL
The Journal of infectious diseases 2004 Jul 15;190(2):236-45

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Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.

Canonico B, Zamai L, Burattini S, Granger V, Mannello F, Gobbi P, Felici C, Falcieri E, Reilly JT, Barnett D, Papa S
Journal of immunological methods 2004 Dec;295(1-2):67-78

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Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Stacchini A, Demurtas A, Godio L, Martini G, Antinoro V, Palestro G
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):10-8

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Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols.

Kappelmayer J, Gratama JW, Karászi E, Menéndez P, Ciudad J, Rivas R, Orfao A
Journal of immunological methods 2000 Aug 28;242(1-2):53-65

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Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.

Paz-Miguel JE, Flores R, Sánchez-Velasco P, Ocejo-Vinyals G, Escribano de Diego J, López de Rego J, Leyva-Cobián F
Journal of immunology (Baltimore, Md. : 1950) 1999 Nov 15;163(10):5399-410

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T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.

Cron RQ, Bort SJ, Wang Y, Brunvand MW, Lewis DB
Journal of immunology (Baltimore, Md. : 1950) 1999 Jan 15;162(2):860-70

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained using FITC of anti-human CD3 monoclonal antibody (clone S4.1). The negative control profiles represent unstained cells.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Polyreactivity Is Inherent to Naive B Cells Selected into the Broadly Neutralizing Response (A) Proportion of mAbs generated from total naive B cells and MBCs and from influenza stalk domain-binding germline B cells, MBCs, and plasmablasts that were polyreactive. (B and C) Binding AUC of polyreactive mAbs or the corresponding germline-reverted mAbs binding to A/California/7/2009 (n = 12) and A/Brisbane/59/2007 viruses (n = 13) (B) and dsDNA (n = 11), insulin (n = 12), and LPS (n = 12) (C). Each line connects the germline and affinity-matured version of the same mAb. (D and E) Fold change in AUC of affinity-matured mAbs over AUC of germline mAbs of all tested mAbs (D) and further broken down by initial germline affinity for A/California/7/2009 virus (E). Cyan dots represent sc70 1F02, and orange dots represent SFV005 2G02. (F and G) MD simulations of the HC-CDR3 sequences of germline and affinity-matured versions of mAbs sc70 1F02 (F) and SFV005 2G02 (G). For (A), the numbers on top of individual bars is the number of polyreactive mAbs out of total mAbs tested from each cohort. For (D) and (E), each symbol represents one mAb, and the red bar represents the median. Data in (A) were analyzed by Fisher's exact test, and data in (B) and (C) were analyzed by paired non-parametric Wilcoxon matched-pairs signed rank tests. See also Figure S6 .

Conjugate

Green dye