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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- MHCD0320 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3 Monoclonal Antibody (7D6), Alexa Fluor™ 488
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 7D6
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues.
Ñahui Palomino RA, Vanpouille C, Laghi L, Parolin C, Melikov K, Backlund P, Vitali B, Margolis L
Nature communications 2019 Dec 11;10(1):5656
Nature communications 2019 Dec 11;10(1):5656
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained using Alexa Fluor® 488 of anti-human CD3 monoclonal antibody (clone S4.1). The negative control profiles represent unstained cells.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Viability of cells treated with Lactobacillus -derived EVs. MT-4 and Jurkat-tat cells were treated or not treated for 3 days with EVs (5 x 10 8 EVs per mL) isolated from four Lactobacillus strains ( L. crispatus BC3, L. crispatus BC5, L. gasseri BC12, L. gasseri BC13), and with particles derived from MRS medium. Triton X-100 (0.2%) was used as a cytotoxic agent. a The numbers of viable cells were counted according to a propidium-iodide-based assay. Results are expressed as percentages of viable cells in EV-free or EV-treated cells. Presented are means +- SEM from five independent measurements. b Cell viability according to MTT assay. Results are expressed as percentages of viable cells in EV-free or EV-treated cells. Presented are means +- SEM from five independent measurements. c Cell depletion in MT-4 cell cultures treated with L. gasseri BC12-derived EVs (5 x 10 8 EVs per mL), as measured with flow cytometry. Panels from left to right represent staining for live/dead cells, CD3, and CD4 populations in EV-untreated cells (upper row) and in cells treated with Lactobacillus- derived EVs (lower row). Asterisks indicate statistical significance by one-way ANOVA multiple comparison with Dunnett's correction (**** p < 0.0001). Source data are provided as a Source Data file.
- Conjugate
- Green dye
