MA5-42943

antibody from Invitrogen Antibodies

Targeting: CD3D T3D

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42943

Provider

Invitrogen Antibodies

Product name

CD3d Recombinant Rabbit Monoclonal Antibody (2R1O10)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: Jurkat. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein. Immunogen sequence: RCNGTDIYKD KESTVQVHYR MCQSCVELDP ATVAGIIVTD VIATLLLALG VFCFAGHETG RLSGAADTQA LLRNDQVYQP LRDRDDAQYS HLGGNWARNK

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

2R1O10

Vial size

100 µL

Concentration

0.51 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD3d in extracts of Jurkat cells. Samples were incubated with CD3d Monoclonal antibody (Product # MA5-42943) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CD3d in mouse spleen using a CD3d Recombinant Monoclonal antibody (Product # MA5-42943). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of CD3d in mouse spleen. Samples were incubated with CD3d Monoclonal antibody (Product # MA5-42943) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of CD3d in rat spleen. Samples were incubated with CD3d Monoclonal antibody (Product # MA5-42943) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of CD3d in rat spleen. Samples were incubated with CD3d Monoclonal antibody (Product # MA5-42943) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of CD3d in Jurkat cells using a CD3d Recombinant Monoclonal antibody (Product # MA5-42943). The Jurkat cells were treated with a Rabbit IgG isotype control (10 µg/mL, blue line) or the CD3D Rabbit mAb (2.5 µg/mL orange line). This was followed by a goat anti-Rabbit FITC conjugated (1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as a blank control (red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of CD3d in Jurkat cells using a CD3d Recombinant Monoclonal antibody (Product # MA5-42943). The Jurkat cells were treated with a Rabbit IgG isotype control (10 µg/mL, blue line) or the CD3D Rabbit mAb (2.5 µg/mL orange line). This was followed by a goat anti-Rabbit FITC conjugated (1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as a blank control (red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis option of CD3d in Jurkat cells. Cells were stained with Rabbit IgG isotype control (2.5 μg/mL, blue line) or CD3d monoclonal antibody (Product # MA5-42943) (2.5 μg/mL, orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line).