Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-14433 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3/CD20 Antibody Cocktail
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-14433 targets CD3/CD20 in IHC (P) and WB applications and shows reactivity with Human, Mouse and Rat samples. CD3 (clone SP7) is a rabbit monoclonal IgG antibody. CD20 (clone L26) is an IgG2a mouse monoclonal antibody. The MA5-14433 immunogen is synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon chain of human CD3 protein; the CD20 immunogen is human tonsil B cells.
- Reactivity
- Human, Mouse, Rat
- Isotype
- IgG
- Antibody clone number
- CD3-SP7; CD20-L26
- Vial size
- 7 mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD3/CD20 was performed by loading 25 µg of Raji (lane 1), Ramos (lane 2) and BAF-3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD3/CD20 monoclonal antibody (Product # MA5-14433) at a dilution of 1:1 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~33 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD3/CD20 was performed by loading 25 µg of CEM (lane 1), Jurkat (lane 2) and rat thymus (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD3/CD20 monoclonal antibody (Product # MA5-14433) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~19 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded Human Tonsil stained with the MultiVision anti-Rabbit/ HRP + anti-Mouse/ AP.Note: Cell membrane staining of CD3 (in red) and CD20 (blue).