- 37-4800 - Invitrogen Antibodies CLDN7 antibody

Submitted references CYP1B1 Augments the Mesenchymal, Claudin-Low, and Chemoresistant Phenotypes of Triple-Negative Breast Cancer Cells.

Hollis PR, Mobley RJ, Bhuju J, Abell AN, Sutter CH, Sutter TR
International journal of molecular sciences 2022 Aug 26;23(17)

Adherens junction regulates cryptic lamellipodia formation for epithelial cell migration.

Ozawa M, Hiver S, Yamamoto T, Shibata T, Upadhyayula S, Mimori-Kiyosue Y, Takeichi M
The Journal of cell biology 2020 Oct 5;219(10)

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Ahmad R, Rah B, Bastola D, Dhawan P, Singh AB
Scientific reports 2017 Jul 11;7(1):5125

Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasis.

Wu CJ, Feng X, Lu M, Morimura S, Udey MC
The Journal of clinical investigation 2017 Feb 1;127(2):623-634

Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers.

Watari A, Hashegawa M, Yagi K, Kondoh M
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2015 Jan;89:232-8

A CLDN1-negative phenotype predicts poor prognosis in triple-negative breast cancer.

Ma F, Ding X, Fan Y, Ying J, Zheng S, Lu N, Xu B
PloS one 2014;9(11):e112765

Rab25 regulates integrin expression in polarized colonic epithelial cells.

Krishnan M, Lapierre LA, Knowles BC, Goldenring JR
Molecular biology of the cell 2013 Mar;24(6):818-31

Utility of immunohistochemical analysis of KAI1, epithelial-specific antigen, and epithelial-related antigen for distinction of chromophobe renal cell carcinoma, an eosinophilic variant from renal oncocytoma.

Ohe C, Kuroda N, Takasu K, Senzaki H, Shikata N, Yamaguchi T, Miyasaka C, Nakano Y, Sakaida N, Uemura Y
Medical molecular morphology 2012 Jun;45(2):98-104

Franceschetti hereditary recurrent corneal erosion.

Lisch W, Bron AJ, Munier FL, Schorderet DF, Tiab L, Lange C, Saikia P, Reinhard T, Weiss JS, Gundlach E, Pleyer U, Lisch C, Auw-Haedrich C
American journal of ophthalmology 2012 Jun;153(6):1073-81.e4

Establishment of a human conjunctival epithelial cell line lacking the functional TACSTD2 gene (an American Ophthalmological Society thesis).

Kinoshita S, Kawasaki S, Kitazawa K, Shinomiya K
Transactions of the American Ophthalmological Society 2012 Dec;110:166-77

Downregulation of cell surface CA125/MUC16 induces epithelial-to-mesenchymal transition and restores EGFR signalling in NIH:OVCAR3 ovarian carcinoma cells.

Comamala M, Pinard M, Thériault C, Matte I, Albert A, Boivin M, Beaudin J, Piché A, Rancourt C
British journal of cancer 2011 Mar 15;104(6):989-99

Cluster analysis of immunohistochemical profiles delineates CK7, vimentin, S100A1 and C-kit (CD117) as an optimal panel in the differential diagnosis of renal oncocytoma from its mimics.

Carvalho JC, Wasco MJ, Kunju LP, Thomas DG, Shah RB
Histopathology 2011 Jan;58(2):169-79

Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.

Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Tsujikawa M, Tanioka H, Nagata-Takaoka M, Hamuro J, Kinoshita S
The American journal of pathology 2010 Sep;177(3):1344-55

Claudin 1 and Claudin 7 Gene Polymorphisms and Protein Derangement are Unrelated to the Growth Pattern and Tumor Volume of Colon Carcinoma.

Victoria HS, Henrik E, Lennart B, Lennart F
International journal of biomedical science : IJBS 2010 Jun;6(2):96-102

Differential expression of claudin tight junction proteins in the human cortical nephron.

Kirk A, Campbell S, Bass P, Mason J, Collins J
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19

Claudin-7 expression in human epithelial ovarian cancer.

Tassi RA, Bignotti E, Falchetti M, Ravanini M, Calza S, Ravaggi A, Bandiera E, Facchetti F, Pecorelli S, Santin AD
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2008 Nov-Dec;18(6):1262-71

Reduced expression of Claudin-7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease.

Sauer T, Pedersen MK, Ebeltoft K, Naess O
Cytopathology : official journal of the British Society for Clinical Cytology 2005 Aug;16(4):193-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of COLO205 (lane 1), HCT 116 (lane 2), HT-29 (lane 3) and Mouse Pancreas (lane 4). The blots were probed with Anti-Claudin-7 Mouse Monoclonal Antibody (Product # 37-4800, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). Bands at 22 and 16 kDa corresponding to Claudin-7 was observed across cell lines tested, except HCT 116. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Claudin 7 Monoclonal Antibody (5D10F3) (Product # 37-4800) and a 22 kDa band corresponding to CLDN7 was observed in OVCAR-3, HT-29, and HCT 116 and not in SK-O-V3, Raji and Jurkat which are reported to be less expressing for CLDN7. An uncharacterized band (*) at ~36 kDa was also observed in the samples tested. Membrane enriched extracts (30 µg lysate) of SK-O-V3 (Lane 1), OVCAR-3 (Lane 2), HT-29 (Lane 3), HCT 116 (Lane 4), Raji (Lane 5) and Jurkat (Lane 6) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Claudin-7 was done on 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Claudin-7 (5D10F3) Mouse Monoclonal Antibody (Product # 37-4800) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300).Panel d is a merged image showing cell junction localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin-7 showing staining in the cytoplasm and membrane of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Claudin-7 monoclonal antibody (Product # 37-4800) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin-7 showing staining in the cytoplasm and membrane of paraffin-embedded human prostate tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Claudin-7 monoclonal antibody (Product # 37-4800) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin-7 showing staining in the cytoplasm and membrane of paraffin-embedded mouse colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Claudin-7 monoclonal antibody (Product # 37-4800) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 6: Rab25 depletion alters the transepithelial resistance and claudin expression. (A) TER was measured in all cell lines from day 3 to day 15 on Transwell filters. During this period, the media were changed every day, and TER was measured in 24-well Transwell inserts. Results are expressed in ohm-cm 2 and are representative of three separate experiments. (B) Equal amounts of total protein lysates (20 mug) from Control, Rab25KD, and Rescue cells were analyzed by Western blots for tight junction proteins. The blots were subsequently stripped and reprobed for beta-actin as a loading control. The results are representative of three separate experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 CA125/MUC16 knockdown disrupts cell-cell junctions. ( A ) Assessment of cell-cell junctions in Ctrl scFv and CA125/MUC16 knockdown cells and OSE cells by electronic microscopy. Arrows indicate the presence of three desmosome spots in Ctrl scFv cells and the lack of such desmosomes in knockdown cells (scale bar - 100 nm). ( B ) Immunofluorescence analysis of claudin-7 expression, a protein involved in tight junctions, in Ctrl scFv and knockdown cells showing the disruption of the protein at the cell surface of knockdown cells ( x 1000 magnification).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Photomicrographs of normal colon mucosa (a and c) and colon carcinoma (b and d) stained immunohistochemically for Claudin 1 (a and b) and Claudin 7 (c and d). An intense staining of the membrane and cytoplasm is seen in the normal mucosa while a weaker staining is seen in tumor cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure S6. Effect of E-cadherin deletion on other proteins. (A) Effect of E-cadherin deletion on p120-catenin level. Wild-type and E-cad KO Caco2 cells were mixed and coimmunostained for E-cadherin (E-cad) and p120-catenin, which allows direct comparison of the level of these proteins between the two cell populations. Graph, relative immunostaining intensity of p120-catenin. A few points in each of the bicellular junctions were randomly selected for measurement, using six wild-type and seven E-cad KO cells. ****, P < 0.0001 ( t test, one-sided). (B) Coimmunostaining for ZO-1 and claudin 7 or 34/ARPC2 and actin in wild-type and E-cad KO Caco2 cells. Scale bars, 10 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 CYP1B1 suppression is associated with increased expression of claudins in CL triple-negative breast cancer (TNBC) cells. ( A ) qPCR analysis of CLDN1, CLDN3, CLDN4, and CLDN7 transcripts. Values are the mean +- SD, n = 3, relative to CV, set at a value of 1.0. The * indicates a significant difference ( p

37-4800

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