37-4800

antibody from Invitrogen Antibodies

Targeting: CLDN7 CEPTRL2, CPETRL2, Hs.84359

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

37-4800

Provider

Invitrogen Antibodies

Product name

Claudin 7 Monoclonal Antibody (5D10F3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

5D10F3

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

CYP1B1 Augments the Mesenchymal, Claudin-Low, and Chemoresistant Phenotypes of Triple-Negative Breast Cancer Cells.

Hollis PR, Mobley RJ, Bhuju J, Abell AN, Sutter CH, Sutter TR
International journal of molecular sciences 2022 Aug 26;23(17)

---

Adherens junction regulates cryptic lamellipodia formation for epithelial cell migration.

Ozawa M, Hiver S, Yamamoto T, Shibata T, Upadhyayula S, Mimori-Kiyosue Y, Takeichi M
The Journal of cell biology 2020 Oct 5;219(10)

---

Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

Ahmad R, Rah B, Bastola D, Dhawan P, Singh AB
Scientific reports 2017 Jul 11;7(1):5125

---

Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasis.

Wu CJ, Feng X, Lu M, Morimura S, Udey MC
The Journal of clinical investigation 2017 Feb 1;127(2):623-634

---

Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers.

Watari A, Hashegawa M, Yagi K, Kondoh M
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2015 Jan;89:232-8

---

A CLDN1-negative phenotype predicts poor prognosis in triple-negative breast cancer.

Ma F, Ding X, Fan Y, Ying J, Zheng S, Lu N, Xu B
PloS one 2014;9(11):e112765

---

Rab25 regulates integrin expression in polarized colonic epithelial cells.

Krishnan M, Lapierre LA, Knowles BC, Goldenring JR
Molecular biology of the cell 2013 Mar;24(6):818-31

---

Utility of immunohistochemical analysis of KAI1, epithelial-specific antigen, and epithelial-related antigen for distinction of chromophobe renal cell carcinoma, an eosinophilic variant from renal oncocytoma.

Ohe C, Kuroda N, Takasu K, Senzaki H, Shikata N, Yamaguchi T, Miyasaka C, Nakano Y, Sakaida N, Uemura Y
Medical molecular morphology 2012 Jun;45(2):98-104

---

Franceschetti hereditary recurrent corneal erosion.

Lisch W, Bron AJ, Munier FL, Schorderet DF, Tiab L, Lange C, Saikia P, Reinhard T, Weiss JS, Gundlach E, Pleyer U, Lisch C, Auw-Haedrich C
American journal of ophthalmology 2012 Jun;153(6):1073-81.e4

---

Establishment of a human conjunctival epithelial cell line lacking the functional TACSTD2 gene (an American Ophthalmological Society thesis).

Kinoshita S, Kawasaki S, Kitazawa K, Shinomiya K
Transactions of the American Ophthalmological Society 2012 Dec;110:166-77

---

Downregulation of cell surface CA125/MUC16 induces epithelial-to-mesenchymal transition and restores EGFR signalling in NIH:OVCAR3 ovarian carcinoma cells.

Comamala M, Pinard M, Thériault C, Matte I, Albert A, Boivin M, Beaudin J, Piché A, Rancourt C
British journal of cancer 2011 Mar 15;104(6):989-99

---

Cluster analysis of immunohistochemical profiles delineates CK7, vimentin, S100A1 and C-kit (CD117) as an optimal panel in the differential diagnosis of renal oncocytoma from its mimics.

Carvalho JC, Wasco MJ, Kunju LP, Thomas DG, Shah RB
Histopathology 2011 Jan;58(2):169-79

---

Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.

Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Tsujikawa M, Tanioka H, Nagata-Takaoka M, Hamuro J, Kinoshita S
The American journal of pathology 2010 Sep;177(3):1344-55

---

Claudin 1 and Claudin 7 Gene Polymorphisms and Protein Derangement are Unrelated to the Growth Pattern and Tumor Volume of Colon Carcinoma.

Victoria HS, Henrik E, Lennart B, Lennart F
International journal of biomedical science : IJBS 2010 Jun;6(2):96-102

---

Differential expression of claudin tight junction proteins in the human cortical nephron.

Kirk A, Campbell S, Bass P, Mason J, Collins J
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19

---

Claudin-7 expression in human epithelial ovarian cancer.

Tassi RA, Bignotti E, Falchetti M, Ravanini M, Calza S, Ravaggi A, Bandiera E, Facchetti F, Pecorelli S, Santin AD
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2008 Nov-Dec;18(6):1262-71

---

Reduced expression of Claudin-7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease.

Sauer T, Pedersen MK, Ebeltoft K, Naess O
Cytopathology : official journal of the British Society for Clinical Cytology 2005 Aug;16(4):193-8

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Claudin-7 was done on 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Claudin-7 (5D10F3) Mouse Monoclonal Antibody (Product # 37-4800) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300).Panel d is a merged image showing cell junction localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 6: Rab25 depletion alters the transepithelial resistance and claudin expression. (A) TER was measured in all cell lines from day 3 to day 15 on Transwell filters. During this period, the media were changed every day, and TER was measured in 24-well Transwell inserts. Results are expressed in ohm-cm 2 and are representative of three separate experiments. (B) Equal amounts of total protein lysates (20 mug) from Control, Rab25KD, and Rescue cells were analyzed by Western blots for tight junction proteins. The blots were subsequently stripped and reprobed for beta-actin as a loading control. The results are representative of three separate experiments.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 CA125/MUC16 knockdown disrupts cell-cell junctions. ( A ) Assessment of cell-cell junctions in Ctrl scFv and CA125/MUC16 knockdown cells and OSE cells by electronic microscopy. Arrows indicate the presence of three desmosome spots in Ctrl scFv cells and the lack of such desmosomes in knockdown cells (scale bar - 100 nm). ( B ) Immunofluorescence analysis of claudin-7 expression, a protein involved in tight junctions, in Ctrl scFv and knockdown cells showing the disruption of the protein at the cell surface of knockdown cells ( x 1000 magnification).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Photomicrographs of normal colon mucosa (a and c) and colon carcinoma (b and d) stained immunohistochemically for Claudin 1 (a and b) and Claudin 7 (c and d). An intense staining of the membrane and cytoplasm is seen in the normal mucosa while a weaker staining is seen in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure S6. Effect of E-cadherin deletion on other proteins. (A) Effect of E-cadherin deletion on p120-catenin level. Wild-type and E-cad KO Caco2 cells were mixed and coimmunostained for E-cadherin (E-cad) and p120-catenin, which allows direct comparison of the level of these proteins between the two cell populations. Graph, relative immunostaining intensity of p120-catenin. A few points in each of the bicellular junctions were randomly selected for measurement, using six wild-type and seven E-cad KO cells. ****, P < 0.0001 ( t test, one-sided). (B) Coimmunostaining for ZO-1 and claudin 7 or 34/ARPC2 and actin in wild-type and E-cad KO Caco2 cells. Scale bars, 10 um.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 CYP1B1 suppression is associated with increased expression of claudins in CL triple-negative breast cancer (TNBC) cells. ( A ) qPCR analysis of CLDN1, CLDN3, CLDN4, and CLDN7 transcripts. Values are the mean +- SD, n = 3, relative to CV, set at a value of 1.0. The * indicates a significant difference ( p