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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA1-37474 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
- Concentration
- 0.187 mg/mL
Submitted references Immunoexpression of claudins 4 and 7 among invasive breast carcinoma subtypes: A large diagnostic study using tissue microarray.
Logullo AF, Pasini FS, Nonogaki S, Rocha RM, Soares FA, Brentani MM
Molecular and clinical oncology 2018 Oct;9(4):377-388
Molecular and clinical oncology 2018 Oct;9(4):377-388
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Claudin 7 Polyclonal Antibody (Product # PA5-32356) and a 23 kDa band corresponding to CLDN7 was observed across all the cell lines and tissues tested except Mouse and Rat Heart. Membrane enriched extracts (30 µg lysate) of HT-29 (Lane 1), HCT 116 (Lane 2), Caco-2 (Lane 3), Panc-1 (Lane 4), tissue extracts (30 ug lysate) of Mouse Colon (Lane 5), Mouse Heart (Lane 6), Rat Colon (Lane 7) and Rat Heart (Lane 8) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Claudin-7 using anti-Claudin-7 Polyclonal Antibody (Product # PA5-32356) in Colon Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Claudin 7 using a polyclonal antibody (Product # PA1-37474).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Examples of CD24, CD44 and claudin 4 and 7 immunostaining in mammary invasive carcinomas (scale bar, 100 um). (A) Claudin-negative; (B) CD24-negative; (C) CD44-negative; (D) claudin 4 2+; (E) claudin 7 2+; (F) CD24-positive; (G) claudin 4 3+; (H) claudin 7 3+; (I) CD44-positive.
