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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-32356 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon carcinoma.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 0.12 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Immunoexpression of claudins 4 and 7 among invasive breast carcinoma subtypes: A large diagnostic study using tissue microarray.
Logullo AF, Pasini FS, Nonogaki S, Rocha RM, Soares FA, Brentani MM
Molecular and clinical oncology 2018 Oct;9(4):377-388
Molecular and clinical oncology 2018 Oct;9(4):377-388
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Claudin 7 Polyclonal Antibody (Product # PA5-32356) and a 23 kDa band corresponding to CLDN7 was observed across all the cell lines and tissues tested except Mouse and Rat Heart. Membrane enriched extracts (30 µg lysate) of HT-29 (Lane 1), HCT 116 (Lane 2), Caco-2 (Lane 3), Panc-1 (Lane 4), tissue extracts (30 ug lysate) of Mouse Colon (Lane 5), Mouse Heart (Lane 6), Rat Colon (Lane 7) and Rat Heart (Lane 8) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Claudin-7 using anti-Claudin-7 Polyclonal Antibody (Product # PA5-32356) in Colon Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Claudin 7 using a polyclonal antibody (Product # PA1-37474).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Examples of CD24, CD44 and claudin 4 and 7 immunostaining in mammary invasive carcinomas (scale bar, 100 um). (A) Claudin-negative; (B) CD24-negative; (C) CD44-negative; (D) claudin 4 2+; (E) claudin 7 2+; (F) CD24-positive; (G) claudin 4 3+; (H) claudin 7 3+; (I) CD44-positive.
