LS-B15920

antibody from LSBio

Targeting: CDH11 CAD11, OB

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B15920

Provider

LSBio

Product name

IHC-plus™ CDH11 / Cadherin 11 Antibody (aa637-796) LS-B15920

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: CDH11 antibody at 6 ug/ml+Jurkat whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 88 kDa. Observed band size: 88 kDa.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: CDH11 antibody at 6µg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 88, 77 kDa Observed band size: 88 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating CDH11 in SH-SY5Y whole cell lysate Lane 1: Rabbit control IgG instead of CDH11 Antibody in SH-SY5Y whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CDH11 Antibody (8µg) + SH-SY5Y whole cell lysate (500µg) Lane 3: SH-SY5Y whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CDH11 in SH-SY5Y whole cell lysate Lane 1: Rabbit control IgG instead of CDH11 Antibody in SH-SY5Y whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CDH11 Antibody (8µg) + SH-SY5Y whole cell lysate (500µg) Lane 3: SH-SY5Y whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CDH11 in SH-SY5Y whole cell lysate Lane 1: Rabbit control IgG instead of CDH11 Antibody in SH-SY5Y whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CDH11 Antibody (8µg) + SH-SY5Y whole cell lysate (500µg) Lane 3: SH-SY5Y whole cell lysate (20µg)

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at 1:100 dilution.