AF1790

antibody from R&D Systems

Targeting: CDH11 CAD11, OB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF1790

Provider

R&D Systems

Product name

Human Cadherin-11 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Cadherin-11 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-7, rhCadherin-8, rhCadherin-10, rhCadherin-18 and rhCadherin-20 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAA35622


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

N-cadherin-mediated cell-cell adhesion promotes cell migration in a three-dimensional matrix.

Shih W, Yamada S
Journal of cell science 2012 Aug 1;125(Pt 15):3661-70

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Cadherin-11 promotes the metastasis of prostate cancer cells to bone.

Chu K, Cheng CJ, Ye X, Lee YC, Zurita AJ, Chen DT, Yu-Lee LY, Zhang S, Yeh ET, Hu MC, Logothetis CJ, Lin SH
Molecular cancer research : MCR 2008 Aug;6(8):1259-67

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Secretion and organization of a cornea-like tissue in vitro by stem cells from human corneal stroma.

Du Y, Sundarraj N, Funderburgh ML, Harvey SA, Birk DE, Funderburgh JL
Investigative ophthalmology & visual science 2007 Nov;48(11):5038-45

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Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.

De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
Biotechnology and bioengineering 2007 Aug 15;97(6):1617-25

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E-cadherin cell-cell adhesion in ewing tumor cells mediates suppression of anoikis through activation of the ErbB4 tyrosine kinase.

Kang HG, Jenabi JM, Zhang J, Keshelava N, Shimada H, May WA, Ng T, Reynolds CP, Triche TJ, Sorensen PH
Cancer research 2007 Apr 1;67(7):3094-105

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Cadherin-11 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cadherin-11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cadherin-11 in Human Prostate Cancer Tissue. Cadherin-11 was detected in fomralin fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Cadherin-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized in the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.