12929-2-AP

antibody from Proteintech Group

Targeting: SRSF1 ASF, MGC5228, SF2, SF2p33, SFRS1, SRp30a

Provider product page for 12929-2-AP

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Antibody data

Antibody Data
Antigen structure
References [50]
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Validations
Western blot [1]

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Product number: 12929-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12929-2-AP, RRID:AB_2187211
Product name: ASF/SF2 antibody
Antibody type: Polyclonal
Description: KD/KO validated ASF/SF2 antibody (Cat. #12929-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SRSF1 protein structure - 12929-2-AP shown in red.

Submitted references Inhibition of PRMT5 triggers synthetic lethality in ARID1A-deficient endometrial cancer by promoting aberrant R-loop accumulation.

Shu W, Dong K, Shen X, Zhou X, Zhang J, Yu S, Cheng S, Zhang T, Chen G, Zhong G, Zhang J, Wang H
Molecular cancer 2026 Mar 9;25(1)

LncRNA-splicing factor condensates regulate hypoxia-responsive pre-mRNA processing near nuclear speckles.

Song YJ, Shinn MK, Bangru S, Wang Y, Sun Q, Hao Q, Chaturvedi P, Freier SM, Perez-Pinera P, Nelson ER, Belmont AS, Guttman M, Prasanth SG, Kalsotra A, Pappu RV, Prasanth KV
Molecular cell 2026 Mar 19;86(6):1061-1080.e10

SRSF1 as a promising biomarker and key player in the pathogenesis of Alzheimer's disease.

Liu Y, Zhao Y, Yao Y, Liu D, Sun Y, Xu W, Yu H, Chi L
Molecular medicine reports 2026 Jun;33(6)

Ultra-low-input rG4-seq reveals the RNA G-quadruplex regulome in gene expression and genome integrity.

Meng TG, Yue W, Li C, Gao D, Du Z, Yi LT, Liu SC, Xu K, Lu YH, Ma XS, Guo JN, Zhou CY, Ju Z, Luo SM, Su R, Ou XH, Sun QY
Nucleic acids research 2026 Jan 22;54(3)

Landscape of splicing factors in early-onset gastric cancer reveals SRSF1 as a key driver of oxaliplatin resistance.

Zhong B, Xiong Z, Xu H, Zheng J, Mohamed SA, Sun J, Huang D, Deng Z, Guo J, Peng J, Chen Y, Lian L
The Journal of biological chemistry 2026 Apr;302(4):111279

Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma.

Xu M, Zheng Y, Zhao L, Wang S, Chen J, Fu Z, Ma A, Liang B, Huang Y, Xu H, Wang Y, Ai Y, Zeng H, Gao C, Cai J, Ke A, Fan J
Signal transduction and targeted therapy 2026 Apr 5;11(1)

Divergent splicing factor SRSF1 signaling promotes inflammation post-CME: the SRSF1/ENPP3 axis acts via inhibition of BRD4 O-GlcNAcylation to enhance NF-κB activation and accelerate heart failure.

Hu CK, He L, Huang WZ, Huang Y, Dai RX, Chang C, Qiu JX, Wu Q, Su Q
Theranostics 2025;15(14):6839-6856

The PIWI-interacting RNA CRAPIR alleviates myocardial ischemia‒reperfusion injury by reducing p53-mediated apoptosis via binding to SRSF1.

Yan H, Li H, Yin DH, Zhang ZZ, Zhang QY, Ren ZY, Hu Y, Zheng GY, Liu Y, Ma WY, Liu YN, Wang XX, Cai BZ, Chen HY
Acta pharmacologica Sinica 2025 Sep;46(9):2393-2406

The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.

Wang Y, Zhao D, Lu J, Hou N, Wu Q, Zhou S, Xu J, Xue W, Cui W, Zheng J, Wang F, Zhai W
Oncogene 2025 Sep;44(36):3310-3332

Dynamic U2AF cycling defines two phases of cotranscriptional pre-mRNA splicing.

Shao C, Hao Y, Jiang L, Wang D, Wang R, Li Y, Wang H, Ge Y, Bai R, Du X, Chen X, Wu T, Gou LT, Wan R, Xu Y, Ji X, Fu XD
Science (New York, N.Y.) 2025 Sep 25;389(6767):eadj9141

RNA splicing is required for timely completion of abscission and is modulated by the abscission checkpoint.

Couldwell GC, Vincent-Mueller JN, Mackay DR, Jensen CC, Stubben CJ, Li L, Tan AC, Sundquist WI, Ullman KS
bioRxiv : the preprint server for biology 2025 Nov 11;

SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program.

Ly J, Cady SL, Haug S, Khalizeva E, Cheeseman IM
Molecular biology of the cell 2025 Nov 1;36(11):ar138

SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program.

Ly J, Cady SL, Khalizeva E, Haug S, Cheeseman IM
bioRxiv : the preprint server for biology 2025 Jul 27;

Proteomic study identifies Aurora-A-mediated regulation of alternative splicing through multiple splicing factors.

Damodaran AP, Gavard O, Gagné JP, Rogalska ME, Behera AK, Mancini E, Bertolin G, Courtheoux T, Kumari B, Cailloce J, Mereau A, Poirier GG, Valcárcel J, Gonatopoulos-Pournatzis T, Watrin E, Prigent C
The Journal of biological chemistry 2025 Jan;301(1):108000

LncRNA BBOX1-AS1 Contributes to Laryngeal Carcinoma Progression by Recruiting SRSF1 to Maintain EFNB2 mRNA Stability.

Zhu X, Li X, Zhang M, Ni J
Biochemical genetics 2025 Aug;63(4):3380-3398

Subcellular proteomics reveals the crosstalk between nucleocytoplasmic trafficking and the innate immune response to Senecavirus A infection.

Li M, Deng X, Zhou M, Wan H, Shi Y, Zhang L, He W, Zhang Y, Hu M, Du Y, Jiang D, Han S, Wan B, Zhang G
International journal of biological macromolecules 2025 Apr;298:139898

LncRNA MAAMT facilitates macrophage recruitment and proinflammatory activation and exacerbates autoimmune myocarditis through the SRSF1/NF-κB axis.

Gan T, Liu W, Wang Y, Huang D, Hu J, Wang Y, Xiong J, Wang X, Xu Q, Xiong N, Lu S, Wang Z
International journal of biological macromolecules 2024 Oct;278(Pt 1):134193

Splicing factor SRSF1 attenuates cardiomyocytes apoptosis via regulating alternative splicing of Bcl2L12.

Xie Y, Yang Z, Chen W, Zhong C, Li M, Zhang L, Cheng T, Deng Q, Wang H, Ju J, Du Z, Liang H
Cell & bioscience 2024 Nov 22;14(1):142

High expression of SRSF1 facilitates osteosarcoma progression and unveils its potential mechanisms.

Li S, Huang X, Zheng S, Zhang W, Liu F, Cao Q
BMC cancer 2024 May 12;24(1):580

Circ_0007432 promotes non-small cell lung cancer progression and macrophage M2 polarization through SRSF1/KLF12 axis.

Mao S, Wu D, Cheng X, Wu J
iScience 2024 Jun 21;27(6):109861

TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression.

Guo L, Liu JJ, Long SY, Wang PY, Li S, Wang JL, Wei XF, Li J, Lei L, Huang AL, Hu JL
Journal of medical virology 2024 Feb;96(2):e29439

Exosome-delivered NR2F1-AS1 and NR2F1 drive phenotypic transition from dormancy to proliferation in treatment-resistant prostate cancer via stabilizing hormonal receptors.

Chen W, Mao Y, Zhan Y, Li W, Wu J, Mao X, Xu B, Shu F
Journal of nanobiotechnology 2024 Dec 18;22(1):761

LncRNA HOTAIR accelerates free fatty acid-induced inflammatory response in HepG2 cells by recruiting SRSF1 to stabilize MLXIPL mRNA.

Guo B, Yan S, Zhai L, Cheng Y
Cytotechnology 2024 Apr;76(2):259-269

SRSF1-mediated alternative splicing is required for spermatogenesis.

Lei WL, Li YY, Du Z, Su R, Meng TG, Ning Y, Hou G, Schatten H, Wang ZB, Han Z, Sun F, Qian WP, Liu C, Sun QY
International journal of biological sciences 2023;19(15):4883-4897

Long-read sequencing reveals the landscape of aberrant alternative splicing and novel therapeutic target in colorectal cancer.

Sun Q, Han Y, He J, Wang J, Ma X, Ning Q, Zhao Q, Jin Q, Yang L, Li S, Li Y, Zhi Q, Zheng J, Dong D
Genome medicine 2023 Sep 21;15(1):76

SRSF1 is essential for primary follicle development by regulating granulosa cell survival via mRNA alternative splicing.

Yao X, Wang C, Yu W, Sun L, Lv Z, Xie X, Tian S, Yan L, Zhang H, Liu J
Cellular and molecular life sciences : CMLS 2023 Oct 31;80(11):343

Targeting PRMT1-mediated SRSF1 methylation to suppress oncogenic exon inclusion events and breast tumorigenesis.

Li WJ, Huang Y, Lin YA, Zhang BD, Li MY, Zou YQ, Hu GS, He YH, Yang JJ, Xie BL, Huang HH, Deng X, Liu W
Cell reports 2023 Nov 28;42(11):113385

RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway.

Feng Z, Ke S, Wang C, Lu S, Xu Y, Yu H, Li Z, Yin B, Li X, Hua Y, Qian B, Bai M, Fu Y, Zhang Y, Wu Y, Ma Y
Oncogene 2023 Jun;42(24):2017-2030

HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway.

Xu Y, Ke S, Lu S, Wang C, Li Z, Feng Z, Yu H, Bai M, Qian B, Yin B, Li X, Hua Y, Jiang H, Ma Y
Oncogenesis 2023 Jan 20;12(1):2

Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction.

Gao X, Li H, Zhang W, Wang X, Sun H, Cao Y, Zhao Y, Ji H, Yang F, Ma W, Liu Y, Yang B, Cai B
International journal of biological sciences 2022;18(7):2980-2993

LncRNA DACH1 protects against pulmonary fibrosis by binding to SRSF1 to suppress CTNNB1 accumulation.

Sun J, Jin T, Niu Z, Guo J, Guo Y, Yang R, Wang Q, Gao H, Zhang Y, Li T, He W, Li Z, Ma W, Su W, Li L, Fan X, Shan H, Liang H
Acta pharmaceutica Sinica. B 2022 Sep;12(9):3602-3617

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells.

Li Q, Yao H, Wang Y, Wu Y, Thorne RF, Zhu Y, Wu M, Liu L
Cell reports 2022 Nov 22;41(8):111707

NSrp70 suppresses metastasis in triple-negative breast cancer by modulating Numb/TβR1/EMT axis.

Zhao Y, Sun H, Zhao Y, Liu Q, Liu Y, Hou Y, Jin W
Oncogene 2022 Jun;41(25):3409-3422

circSLC41A1 Resists Porcine Granulosa Cell Apoptosis and Follicular Atresia by Promoting SRSF1 through miR-9820-5p Sponging.

Wang H, Zhang Y, Zhang J, Du X, Li Q, Pan Z
International journal of molecular sciences 2022 Jan 28;23(3)

Extracellular matrix stiffness controls VEGF(165) secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis.

Bao M, Chen Y, Liu JT, Bao H, Wang WB, Qi YX, Lv F
Angiogenesis 2022 Feb;25(1):71-86

The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.

Sun J, Jin T, Su W, Guo Y, Niu Z, Guo J, Li L, Wang J, Ma L, Yu T, Li X, Zhou Y, Shan H, Liang H
Cell death and differentiation 2021 Oct;28(10):2916-2930

SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity.

Malik I, Tseng YJ, Wright SE, Zheng K, Ramaiyer P, Green KM, Todd PK
EMBO molecular medicine 2021 Nov 8;13(11):e14163

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Khan M, Hou S, Azam S, Lei H
Nucleic acids research 2021 Jun 21;49(11):6420-6436

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.

Tran JR, Paulson DI, Moresco JJ, Adam SA, Yates JR, Goldman RD, Zheng Y
The Journal of cell biology 2021 Jan 4;220(1)

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

Zhao J, Jiang Y, Zhang H, Zhou J, Chen L, Li H, Xu J, Zhang G, Jing Z
Journal of experimental & clinical cancer research : CR 2021 Apr 15;40(1):134

LncRNA HOTAIR aggravates myocardial ischemia-reperfusion injury by sponging microRNA-126 to upregulate SRSF1.

Sun Y, Hu ZQ
European review for medical and pharmacological sciences 2020 Sep;24(17):9046-9054

CD63(+) Cancer-Associated Fibroblasts Confer Tamoxifen Resistance to Breast Cancer Cells through Exosomal miR-22.

Gao Y, Li X, Zeng C, Liu C, Hao Q, Li W, Zhang K, Zhang W, Wang S, Zhao H, Fan D, Li M, Zhang Y, Zhang W, Zhang C
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2020 Nov;7(21):2002518

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA-Binding Proteins and Sponging MiR-942.

Wong CH, Lou UK, Li Y, Chan SL, Tong JH, To KF, Chen Y
Cancer research 2020 Jun 1;80(11):2138-2149

Long noncoding RNA HOXA11-AS promotes gastric cancer cell proliferation and invasion via SRSF1 and functions as a biomarker in gastric cancer.

Liu Y, Zhang YM, Ma FB, Pan SR, Liu BZ
World journal of gastroenterology 2019 Jun 14;25(22):2763-2775

SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.

Raihan O, Brishti A, Li Q, Zhang Q, Li D, Li X, Zhang Q, Xie Z, Li J, Zhang J, Liu Q
Cell reports 2019 Jul 2;28(1):78-90.e6

Defensive effect of microRNA-200b/c against amyloid-beta peptide-induced toxicity in Alzheimer's disease models.

Higaki S, Muramatsu M, Matsuda A, Matsumoto K, Satoh JI, Michikawa M, Niida S
PloS one 2018;13(5):e0196929

Zc3h13 Regulates Nuclear RNA m(6)A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Wen J, Lv R, Ma H, Shen H, He C, Wang J, Jiao F, Liu H, Yang P, Tan L, Lan F, Shi YG, He C, Shi Y, Diao J
Molecular cell 2018 Mar 15;69(6):1028-1038.e6

Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.

Chen Y, Huang Q, Liu W, Zhu Q, Cui CP, Xu L, Guo X, Wang P, Liu J, Dong G, Wei W, Liu CH, Feng Z, He F, Zhang L
Nature communications 2018 Jun 25;9(1):2464

PP1γ functionally augments the alternative splicing of CaMKIIδ through interaction with ASF.

Huang C, Cao W, Liao R, Wang J, Wang Y, Tong L, Chen X, Zhu W, Zhang W
American journal of physiology. Cell physiology 2014 Jan 15;306(2):C167-77

Rescue of cardiac failing and remodelling by inhibition of protein phosphatase 1γ is associated with suppression of the alternative splicing factor-mediated splicing of Ca2+/calmodulin-dependent protein kinase δ.

Liao RJ, Tong LJ, Huang C, Cao WW, Wang YZ, Wang J, Chen XF, Zhu WZ, Zhang W
Clinical and experimental pharmacology & physiology 2014 Dec;41(12):976-85

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Submitted by: Proteintech Group (provider)
Main image
Experimental details: The ASF/SF2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ASF/SF2. This antibody recognizes human,mouse,rat antigen. The ASF/SF2 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 12929-2-AP.pdf

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