AP6857a

antibody from Abgent

Targeting: SRSF1 ASF, MGC5228, SF2, SF2p33, SFRS1, SRp30a

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6857a

Provider

Abgent

Proper citation

Abgent Cat#AP6857a, RRID:AB_10614837

Product name

SFRS1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of SFRS1 Antibody (N-term) (Cat. #AP6857a) in A2058, WiDr cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (N-term) (Cat. #AP6857a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SFRS1 Antibody (N-term) (Cat. #AP6857a) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SFRS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100