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LearnA305-661A-T
antibody from Invitrogen Antibodies
Targeting: SGSM3
DJ1042K10.2, RabGAP-5, RABGAP5, RUSC3, RUTBC3
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- A305-661A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SGSM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SGSM3 by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-SGSM3 antibody (Product # A305-661A-T; lot A305-661A-T-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SGSM3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SGSM3 antibody A305-661A-M (lot A305-661A-M-1) used for IP at 8 µl per reaction. SGSM3 was also immunoprecipitated by rabbit anti-SGSM3 antibody BL22516. For blotting immunoprecipitated SGSM3, A305-661A-M was used at 1:400. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SGSM3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SGSM3 antibody A305-661A-M (lot A305-661A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SGSM3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-SGSM3 antibody (Product # A305-661A-T (lot A305-661A-T-2) used for IP at 20 µL/mg lysate. SGSM3 was also immunoprecipitated by a previous lot of this antibody (lot A305-661A-T-1) and rabbit anti-SGSM3 antibody BL22516. For blotting immunoprecipitated SGSM3, Product # A305-661A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.
