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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AB0034-200 - Provider product page
- Provider
- SICGEN
- Product name
- Anti-Rab11a
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to mouse Rab11b. Rab11 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and plays essential roles in vesicle and granule targeting. Similar to Rab11a, it has been shown that Rab11b associates with recycling endosomes.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- lysates at 100 µg per lane
- Sample type
- At-T20 and HeLa cell lines
- Primary Ab dilution
- 1:2,500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cells were fixed with methanol and permeabilized with 0.1% saponin
- Sample type
- NHI/3T3 cell line
- Primary Ab dilution
- 1:100
- Conjugate
- Unconjugated
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval;
- Sample type
- Human mammary tissue
- Primary Ab dilution
- 1/200
- Conjugate
- Unconjugated
