Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA7-00184-A488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB11A Recombinant Rabbit Monoclonal Antibody (3H18L5), Alexa Fluor™ 488
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, equine, mouse, primate, rat, Xenopus and zebrafish based on sequence homology
- Conjugate
- Green dye
- Antibody clone number
- 3H18L5
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Rab11 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Rab11 Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA7-00184-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Green dye
