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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21430 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL13A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (95%), Rat (96%), Zebrafish (89%), Xenopus laevis (91%), Dog (100%), Pig (98%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.72 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL13A Polyclonal Antibody (Product # PA5-21430). Sample (30 µg of whole cell lysate). A: 293T. B: HeLa. 10% SDS PAGE. RPL13A Polyclonal Antibody (Product # PA5-21430) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPL13A in paraformaldehyde-fixed HeLa cells using a RPL13A polyclonal antibody (Product # PA5-21430) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPL13A antibody (Product # PA5-21430) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using RPL13A (Product # PA5-21430) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
