PAB28454

antibody from Abnova Corporation

Targeting: RBM39 CAPER, CAPERalpha, CC1.3, fSAP59, HCC1, RNPC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28454

Provider

Abnova Corporation

Product name

RBM39 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RBM39.

Antigen sequence

LAAAASVQPLATQCFQLSNMFNPQTEEEVGWDTEI
KDDVIEECNKHGGVIHIYVDKNSAQGNVYVKCPSI
AAAIAAVNALHGRWFAGKMITAAYVPLPTYHNLFP
DSMTATQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: EFO-21, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with RBM39 polyclonal antibody (Cat # PAB28454) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U-2 OS with RBM39 polyclonal antibody (Cat # PAB28454) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli & cytoskeleton (microtubules).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with RBM39 polyclonal antibody (Cat # PAB28454) shows nuclear positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)