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antibody from Invitrogen Antibodies
Targeting: RBM39
CAPER, CAPERalpha, CC1.3, fSAP59, HCC1, RNPC2
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-90681 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: ATQCFQLSNM FNPQTEEEVG WDTEIKDDVI EECNKHGGVI HIYVDKNSAQ GNVYVKCPSI AAAIAAVNAL HGRWFAGKMI TAAYVPLPTY HNLFPDSMTA TQLLVPSRR; Positive Samples: BxPC-3, U-251MG, 293T, LO2, Mouse brain, Mouse heart; Cellular Location: Nucleus speckle
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.66 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HCC1 in extracts of various cell lines. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HCC1 was performed in U-2 OS cells using HCC1 Polyclonal Antibody (Product # PA5-90681).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HCC1 in U-2 OS cells. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCC1 in paraffin-embedded rat liver. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCC1 in paraffin-embedded human colon carcinoma. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCC1 in paraffin-embedded mouse spleen. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCC1 in paraffin-embedded rat liver. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCC1 in paraffin-embedded human colon carcinoma. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCC1 in paraffin-embedded mouse spleen. Samples were incubated with HCC1 Polyclonal antibody (Product # PA5-90681) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
