PA5-31103

antibody from Invitrogen Antibodies

Targeting: RBM39 CAPER, CAPERalpha, CC1.3, fSAP59, HCC1, RNPC2

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31103

Provider

Invitrogen Antibodies

Product name

HCC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, NIH-3T3, JC. Predicted reactivity: Mouse (100%), Rat (97%), Xenopus laevis (83%), Rabbit (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.

Královicová J, Ševcíková I, Stejskalová E, Obuca M, Hiller M, Stanek D, Vorechovský I
Nucleic acids research 2018 Jul 6;46(12):6166-6187

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Exon-centric regulation of ATM expression is population-dependent and amenable to antisense modification by pseudoexon targeting.

Kralovicova J, Knut M, Cross NC, Vorechovsky I
Scientific reports 2016 Jan 6;6:18741

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Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins.

Kralovicova J, Knut M, Cross NC, Vorechovsky I
Nucleic acids research 2015 Apr 20;43(7):3747-63

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. RNA-Seq of HEK293 cells depleted of PUF60 and RBM39. ( A ) Domain structure. ( B ) Western blot analysis of HEK293 cells lacking or overexpressing PUF60 ( left panel ) and lacking RBM39 ( right panel ). hd, homodimers; ex, exogenous; en, endogenous protein; sc, scrambled siRNA controls; EV, empty vector. ( C ) Distribution of RNA processing events altered by depletion of PUF60 and RBM39. Each event was confirmed in the genome browser by visualizing complete transcripts and cleaned APA sites annotated in the APA atlas ( 43 ).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. RBM39 interactions with spliceosome components. ( A ) RBM39 interacts with U1 snRNP and U2AF. Interaction of RBM39 with the U1-specific protein U1-70K, the U2-specific protein U2A' and the small subunit of U2AF was assayed by immunoprecipitations. HeLa cells were transiently transfected with U1-70K-GFP, U2A'-GFP or U2AF35-GFP, immunoprecipitated with anti-GFP antibodies and probed with antibodies shown to the right. U1C and SF3B4 served as positive controls for immunoprecipitations for U1-70K-GFP and U2A'-GFP, respectively. Asterisks denote a partially degraded U2A'-GFP. (B, C) RBM39 interactions monitored by FRET. Cells were transiently co-transfected with RBM39-CFP and C-terminally YFP-tagged U1-70K. ( B ) YFP was bleached in a small region comprising the nucleoplasm and nuclear speckles; CFP fluorescence was measured before and after bleaching. Fluorescence of RBM39 increased after bleaching of U1-70K-YFP [cf. CFP fluorescence in the bleached region (rectangles) before ( top panel ) and after ( bottom panel ) bleaching]. A, acceptor; D, donor; scale bar, 5 mum. ( C ) Quantification of individual donor-acceptor FRET efficiencies upon the inhibition of RNA polymerase II by DRB. Columns indicate means; errors bars SEMs. Interaction between RBM39-CFP and U2AF35-YFP ( 22 ) served as a positive control and interaction between RBM39-CFP and YFP as a negative control. Significantly different means are denoted by an asterisk ( P < 0.01; t -test).