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LearnNB200-333
antibody from Novus Biologicals
Targeting: RBM39
CAPER, CAPERalpha, CC1.3, fSAP59, HCC1, RNPC2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB200-333 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-333, RRID:AB_10000567
- Product name
- Rabbit Polyclonal HCC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references CAPER-α alternative splicing regulates the expression of vascular endothelial growth factor₁₆₅ in Ewing sarcoma cells.
CAPERalpha is a novel Rel-TAD-interacting factor that inhibits lymphocyte transformation by the potent Rel/NF-kappaB oncoprotein v-Rel.
Spatial mapping of splicing factor complexes involved in exon and intron definition.
Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription.
Purification and characterization of cellular proteins associated with histone H4 tails.
Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1.
Proteomic analysis of steady-state nuclear hormone receptor coactivator complexes.
Huang G, Zhou Z, Wang H, Kleinerman ES
Cancer 2012 Apr 15;118(8):2106-16
Cancer 2012 Apr 15;118(8):2106-16
CAPERalpha is a novel Rel-TAD-interacting factor that inhibits lymphocyte transformation by the potent Rel/NF-kappaB oncoprotein v-Rel.
Dutta J, Fan G, Gélinas C
Journal of virology 2008 Nov;82(21):10792-802
Journal of virology 2008 Nov;82(21):10792-802
Spatial mapping of splicing factor complexes involved in exon and intron definition.
Ellis JD, Llères D, Denegri M, Lamond AI, Cáceres JF
The Journal of cell biology 2008 Jun 16;181(6):921-34
The Journal of cell biology 2008 Jun 16;181(6):921-34
Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription.
Kim K, Choi J, Heo K, Kim H, Levens D, Kohno K, Johnson EM, Brock HW, An W
The Journal of biological chemistry 2008 Apr 4;283(14):9113-26
The Journal of biological chemistry 2008 Apr 4;283(14):9113-26
Purification and characterization of cellular proteins associated with histone H4 tails.
Choi J, Kim B, Heo K, Kim K, Kim H, Zhan Y, Ranish JA, An W
The Journal of biological chemistry 2007 Jul 20;282(29):21024-31
The Journal of biological chemistry 2007 Jul 20;282(29):21024-31
Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1.
Meng Q, Rayala SK, Gururaj AE, Talukder AH, O'Malley BW, Kumar R
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 3;104(14):5866-71
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 3;104(14):5866-71
Proteomic analysis of steady-state nuclear hormone receptor coactivator complexes.
Jung SY, Malovannaya A, Wei J, O'Malley BW, Qin J
Molecular endocrinology (Baltimore, Md.) 2005 Oct;19(10):2451-65
Molecular endocrinology (Baltimore, Md.) 2005 Oct;19(10):2451-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HCC1 Antibody [NB200-333] - Whole cell lysate (10 and 50 ug) from HeLa cells. Antibody used at 0.1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HCC1 Antibody [NB200-333] - Detection of human Caper by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caper antibody NB200-333 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HCC1 Antibody [NB200-333] - Detection of human HCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HCC1 antibody NB200-333 (lot 2) used for IP at 6 ug per reaction. HCC1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated HCC1, NB200-333 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HCC1 Antibody [NB200-333] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Caper used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HCC1 Antibody [NB200-333] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.
