Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C313306 - Provider product page
- Provider
- LSBio
- Product name
- GJA4 / CX37 / Connexin 37 Antibody (aa3-17) LS-C313306
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- WB of GJA4 / CX37 / Connexin 37 antibody. Lane 1: Rat Heart Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: NIH3T3 Cell Lysate. Lane 5: SKOV Cell Lysate. Lane 6: A431 Cell Lysate.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Connexin 37/GJA4 using anti-Connexin 37/GJA4 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5; mouse HEPA1-6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: human PANC-1 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 37/GJA4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.05%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Connexin 37/GJA4 at approximately 35-37KD. The expected band size for Connexin 37/GJA4 is at 37KD.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- GJA4 / CX37 / Connexin 37 antibody. IHC(P): Human Intestinal Cancer Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- GJA4 / CX37 / Connexin 37 antibody. IHC(P): Rat Cardiac Muscle Tissue.