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ImmunohistochemistryAntibody data
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- References [5]
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- Immunohistochemistry [4]
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- Product number
- 42-4400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Connexin 37 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- 42-4400 was used in the western blot analysis to successfully detect Connexin 37 in mouse lung homogenate.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Probing Endothelial Cell Mechanics Through Connexin 43 Disruption.
Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells.
NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions.
TIMP-2 mutant decreases MMP-2 activity and augments pressure overload induced LV dysfunction and heart failure.
Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles.
Islam MM, Steward RL Jr
Experimental mechanics 2019 Mar;59(3):327-336
Experimental mechanics 2019 Mar;59(3):327-336
Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells.
Guo R, Si R, Scott BT, Makino A
American journal of physiology. Cell physiology 2017 Apr 1;312(4):C398-C406
American journal of physiology. Cell physiology 2017 Apr 1;312(4):C398-C406
NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions.
Pogoda K, Füller M, Pohl U, Kameritsch P
Cell communication and signaling : CCS 2014 May 15;12:33
Cell communication and signaling : CCS 2014 May 15;12:33
TIMP-2 mutant decreases MMP-2 activity and augments pressure overload induced LV dysfunction and heart failure.
Givvimani S, Kundu S, Narayanan N, Armaghan F, Qipshidze N, Pushpakumar S, Vacek TP, Tyagi SC
Archives of physiology and biochemistry 2013 May;119(2):65-74
Archives of physiology and biochemistry 2013 May;119(2):65-74
Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles.
Simon AM, Chen H, Jackson CL
Cell communication & adhesion 2006 Jan-Apr;13(1-2):61-77
Cell communication & adhesion 2006 Jan-Apr;13(1-2):61-77
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of frozen blood vessels in mouse heart using Zymed Rb anti-Connexin 37 (C-term) (Product # 42-4400). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Connexin 37 showing staining in the cytoplasm and membrane of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Connexin 37 polyclonal antibody (Product # 42-4400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Connexin 37 showing staining in the cytoplasm and membrane of paraffin-embedded mouse lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Connexin 37 polyclonal antibody (Product # 42-4400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Connexin 37 showing staining in the cytoplasm and membrane of paraffin-embedded mouse heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Connexin 37 polyclonal antibody (Product # 42-4400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
