HPA068802

antibody from Atlas Antibodies

Targeting: MED25 ACID1, ARC92, DKFZp434K0512, TCBAP0758

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA068802

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA068802, RRID:AB_2686039

Product name

Anti-MED25

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human MED25, Gene description: mediator complex subunit 25, Alternative Gene Names: ACID1, ARC92, DKFZp434K0512, TCBAP0758, Validated applications: ICC, ChIP, WB, Uniprot ID: Q71SY5, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

An Unexpected Encounter: Respiratory Syncytial Virus Nonstructural Protein 1 Interacts with Mediator Subunit MED25.

Van Royen T, Sedeyn K, Moschonas GD, Toussaint W, Vuylsteke M, Van Haver D, Impens F, Eyckerman S, Lemmens I, Tavernier J, Schepens B, Saelens X
Journal of virology 2022 Oct 12;96(19):e0129722

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-MED25 (HPA068802, Lot 000018373) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.