STJ118590

antibody from St John's Laboratory

Targeting: MED25 ACID1, ARC92, DKFZp434K0512, TCBAP0758

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

STJ118590

Provider

St John's Laboratory

Product name

Anti-MED25 antibody (30-200) (STJ118590)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody anti-MED25 (30-200) is suitable for use in Western Blot and Immunofluorescence.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NA


Epitope

NA

Isotype

IgG

Antibody clone number

NA

Vial size

NA

Concentration

NA

Storage

Store in a freezer at-20°C and avoid freeze-thaw cycles.

Handling

NA

Western blot

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using MED25 antibody (STJ118590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Immunohistochemistry

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunofluorescence analysis of U-2 OS cells using MED25 Polyclonal Antibody (STJ118590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more