PAB13322

antibody from Abnova Corporation

Targeting: ATG12 APG12, APG12L

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB13322

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB13322, RRID:AB_10550512

Product name

ATG12 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ATG12.

Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy.

Hanada T, Noda NN, Satomi Y, Ichimura Y, Fujioka Y, Takao T, Inagaki F, Ohsumi Y
The Journal of biological chemistry 2007 Dec 28;282(52):37298-302

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Autophagy as a cell death and tumor suppressor mechanism.

Gozuacik D, Kimchi A
Oncogene 2004 Apr 12;23(16):2891-906

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Tor-mediated induction of autophagy via an Apg1 protein kinase complex.

Kamada Y, Funakoshi T, Shintani T, Nagano K, Ohsumi M, Ohsumi Y
The Journal of cell biology 2000 Sep 18;150(6):1507-13

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 polyclonal antibody (Cat # PAB13322) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human brain tissue with 2.5 ug/mL ATG12 polyclonal antibody (Cat # PAB13322).

Validation comment

Immunohistochemistry