Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB13322 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB13322, RRID:AB_10550512
- Product name
- ATG12 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATG12.
- Storage
- Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy.
Autophagy as a cell death and tumor suppressor mechanism.
Tor-mediated induction of autophagy via an Apg1 protein kinase complex.
Hanada T, Noda NN, Satomi Y, Ichimura Y, Fujioka Y, Takao T, Inagaki F, Ohsumi Y
The Journal of biological chemistry 2007 Dec 28;282(52):37298-302
The Journal of biological chemistry 2007 Dec 28;282(52):37298-302
Autophagy as a cell death and tumor suppressor mechanism.
Gozuacik D, Kimchi A
Oncogene 2004 Apr 12;23(16):2891-906
Oncogene 2004 Apr 12;23(16):2891-906
Tor-mediated induction of autophagy via an Apg1 protein kinase complex.
Kamada Y, Funakoshi T, Shintani T, Nagano K, Ohsumi M, Ohsumi Y
The Journal of cell biology 2000 Sep 18;150(6):1507-13
The Journal of cell biology 2000 Sep 18;150(6):1507-13
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 polyclonal antibody (Cat # PAB13322) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human brain tissue with 2.5 ug/mL ATG12 polyclonal antibody (Cat # PAB13322).
- Validation comment
- Immunohistochemistry