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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-32180 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, Rat brain, ATG12 shRNA-transfected 293T. Predicted reactivity: Pig (81%), Chimpanzee (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.36 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Atg 12-Atg5 was performed in 293T whole cell extracts using 5 µg of ATG12 Polyclonal Antibody (Product # PA5-32180). Samples were transferred to a membrane and probed with ATG12 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ATG12 was performed in paraffin-embedded human lung cancer tissue using ATG12 Polyclonal Antibody (Product # PA5-32180) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ATG12 was performed in paraffin-embedded rat brain tissue using ATG12 Polyclonal Antibody (Product # PA5-32180) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Atg 12-Atg5 was performed in 293T whole cell extracts using 5 µg of ATG12 Polyclonal Antibody (Product # PA5-32180). Samples were transferred to a membrane and probed with ATG12 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
