Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-32180 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, Rat brain, ATG12 shRNA-transfected 293T.
- Concentration
- 3.08 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG12 using 30 µg of A) 293T and B) A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ATG12 polyclonal antibody (Product # PA5-32180) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG12 in Various whole cell extracts (30 µg). Samples were separated by 12% SDS-PAGE and the membrane was probed with ATG12 Polyclonal antibody (Product # PA5-32180) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATG12 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATG12 Polyclonal Antibody (Product # PA5-32180) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATG12 Polyclonal Antibody (Product # PA5-32180). Sample (50 µg of whole cell lysate). Lane A: Rat brain. 12% SDS PAGE. ATG12 Polyclonal Antibody (Product # PA5-32180) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATG12 Polyclonal Antibody (Product # PA5-32180). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 Polyclonal Antibody (Product # PA5-32180) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATG12 Polyclonal Antibody (Product # PA5-32180). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 12% SDS PAGE. ATG12 Polyclonal Antibody (Product # PA5-32180) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ATG12 was performed in paraffin-embedded rat brain tissue using ATG12 Polyclonal Antibody (Product # PA5-32180) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ATG12 was performed in paraffin-embedded human lung cancer tissue using ATG12 Polyclonal Antibody (Product # PA5-32180) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Atg 12-Atg5 was performed in 293T whole cell extracts using 5 µg of ATG12 Polyclonal Antibody (Product # PA5-32180). Samples were transferred to a membrane and probed with ATG12 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
