39-2800

antibody from Invitrogen Antibodies

Targeting: INCENP FLJ31633

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

39-2800

Provider

Invitrogen Antibodies

Product name

INCENP Monoclonal Antibody (58-217)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

58-217

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Transcription factor Sp1 regulates mitotic chromosome assembly and segregation.

Flashner S, Swift M, Sowash A, Fahmy AN, Azizkhan-Clifford J
Chromosoma 2022 Sep;131(3):175-191

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MKLP2 Is a Motile Kinesin that Transports the Chromosomal Passenger Complex during Anaphase.

Adriaans IE, Hooikaas PJ, Aher A, Vromans MJM, van Es RM, Grigoriev I, Akhmanova A, Lens SMA
Current biology : CB 2020 Jul 6;30(13):2628-2637.e9

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CDK11(p58)-cyclin L1β regulates abscission site assembly.

Renshaw MJ, Panagiotou TC, Lavoie BD, Wilde A
The Journal of biological chemistry 2019 Dec 6;294(49):18639-18649

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Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization.

Unruhe-Knauf B, Knauer SK
Methods in molecular biology (Clifton, N.J.) 2017;1510:47-59

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Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells.

Hindriksen S, Bramer AJ, Truong MA, Vromans MJM, Post JB, Verlaan-Klink I, Snippert HJ, Lens SMA, Hadders MA
PloS one 2017;12(6):e0179514

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Enhanced HSP70 lysine methylation promotes proliferation of cancer cells through activation of Aurora kinase B.

Cho HS, Shimazu T, Toyokawa G, Daigo Y, Maehara Y, Hayami S, Ito A, Masuda K, Ikawa N, Field HI, Tsuchiya E, Ohnuma S, Ponder BA, Yoshida M, Nakamura Y, Hamamoto R
Nature communications 2012;3:1072

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Ser2481-autophosphorylated mTOR colocalizes with chromosomal passenger proteins during mammalian cell cytokinesis.

Vazquez-Martin A, Sauri-Nadal T, Menendez OJ, Oliveras-Ferraros C, Cufí S, Corominas-Faja B, López-Bonet E, Menendez JA
Cell cycle (Georgetown, Tex.) 2012 Nov 15;11(22):4211-21

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The active form of the metabolic sensor: AMP-activated protein kinase (AMPK) directly binds the mitotic apparatus and travels from centrosomes to the spindle midzone during mitosis and cytokinesis.

Vazquez-Martin A, Oliveras-Ferraros C, Menendez JA
Cell cycle (Georgetown, Tex.) 2009 Aug;8(15):2385-98

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Indirect immunofluorescent staining of HeLa cells using Ms anti-INCENP (Product # 39-2800) (green). Nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Indirect immunofluorescent staining of HeLa cells using Ms anti-INCENP (Product # 39-2800) (green). Nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of INCENP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with INCENP Mouse Monoclonal Antibody (Product # 39-2800) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature. Panel a,b,c,d and e represents the merged image showing localization of INCENP in different phases of cell cycle. Panel f shows the no primary antibody control. Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 K561-dimethylated HSP70 interacts with AURKB in the nucleus. ( a ) Cells were treated with 7.5 mug ml -1 of aphidicolin for 24 h to synchronize the cell cycle and stained with anti-HSP70 K561me2, anti-HSP70, anti-AURKB antibodies and DAPI 12 h after release from cell cycle arrest. Scale bar, 20 mum. ( b ) 293T cells were co-transfected with full-length HSP70 and AURKB expression vectors or a mock control vector. The interaction of FLAG-HSP70 with HA-AURKB (upper) or HA-HSP70 with FLAG-AURKB (bottom) was examined by immunoprecipitation using anti-FLAG M2 agarose and immunoblotted with anti-FLAG and anti-HA antibodies. ( c ) FLAG-mock and FLAG-AURKB expression vectors were transfected into 293T cells. After 48 h, cells were immunoprecipitated with anti-FLAG M2 agarose beads, and immunoprecipitants were immunoblotted with anti-FLAG and anti-HSP70 antibodies, respectively. ( d ) HeLa cells were lysed with CelLytic M and immunoprecipitated with normal rabbit IgG (NRIgG) and an anti-AURKB (ab2254, Abcam) antibody. The immunoprecipitates were fractionated by SDS-PAGE and immunoblotted with anti-AURKB (ab2254, Abcam) and anti-HSP70 antibodies (SPA-810, Stressgen). ( e ) Cell lysates from A549 cells were immunoprecipitated with normal mouse IgG (NMIgG) and an anti-HSP70 (ADI-SPA-810, Enzo) antibody. The immunoprecipitates were fractionated by SDS-PAGE and immunoblotted with anti-AURKB (no. 3094, Cell Signaling Technology) and anti-HSP70 (no. 4872, Cell Signaling Technology) a