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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-25669 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLH1 Monoclonal Antibody (G168-15)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested positive controls for this product are tonsil and colon carcinoma.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- G168-15
- Vial size
- 500 µL
- Storage
- 4° C
Submitted references Non-Helicobacter pylori Helicobacter (NHPH) positive gastric cancer.
Yasuda T, Lee HS, Nam SY, Katoh H, Ishibashi Y, Yamagata Murayama S, Matsui H, Masuda H, Rimbara E, Sakurazawa N, Suzuki H, Yoshida H, Seto Y, Ishikawa S, Jeon SW, Nakamura M, Nomura S
Scientific reports 2022 Mar 21;12(1):4811
Scientific reports 2022 Mar 21;12(1):4811
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of human colon carcinoma tissue using (Product # MA1-25669) MLH1 Monoclonal Antibody (G168-15).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 TCGA classification by immunohistochemistry and EBER for the three NHPH positive gastric cancer. ( A , G , M ) Hematoxylin and eosin staining for gastric cancer in Patients 1, 2, and 3, respectively. ( B , H , O ) MLH1 staining was positive for all three gastric cancers and no microsatellite instability was indicated. ( C , I , P ) p53 staining was positive and p53 was aberrantly expressed in all three gastric cancers. ( D , J , Q ) p53 staining in the non-cancerous part of gastric mucosa in each patient. P53 was not aberrantly expressed in normal regions. ( E , K , R ) E-cadherin was positive in all three cancers. ( F , L , S ) EBER in situ hybridization. Epstain-Barr virus was negative in all three stomachs. Inset in ( F ) shows positive control. ( N ) shows the surrounding non-cancerous parts. Taken together, all three gastric cancers were classified into the Chromosomal Instability (CIN) Type.
