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- Antibody Data
- Antigen structure
- References [40]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 11697-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11697-1-AP, RRID:AB_2145604
- Product name
- MLH1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MLH1 antibody (Cat. #11697-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.
DNA methylation mediates the immunosuppressive tumour microenvironment in metastatic endometrial clear cell carcinoma.
Somatic Kitl promotes mTOR to facilitate prophase I of meiosis in female embryonic gonads.
Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta.
Development of Syngeneic Murine Glioma Models with Somatic Mismatch Repair Deficiency to Study Therapeutic Responses to Alkylating Agents and Immunotherapy.
Defect of MLH1 expression sensitized esophageal squamous cell carcinoma cells to Polθ inhibitor.
Aberrant cytoplasmic localization of MLH1 characterizes a cell population that seeds breast cancer recurrence.
Integrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models.
Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane.
The mechanism of RGS5 regulating gastric cancer mismatch repair protein.
TDP43 Interacts with MLH1 and MSH6 Proteins in A DNA Damage-Inducible Manner.
TDP43 interacts with MLH1 and MSH6 proteins in a DNA damage-inducible manner.
Protein disulfide isomerase is essential for spermatogenesis in mice.
Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types.
Maternal Obesity Induces the Meiotic Defects and Epigenetic Alterations During Fetal Oocyte Development.
Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer.
Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination.
The MSH2 c.793-1G>A variant disrupts normal splicing and is associated with Lynch syndrome.
Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small molecules.
Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small molecules.
Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice.
Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.
NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.
Integrated proteogenomic characterization of urothelial carcinoma of the bladder.
Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.
The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.
Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.
C. tropicalis promotes chemotherapy resistance in colon cancer through increasing lactate production to regulate the mismatch repair system.
Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.
Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.
Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.
Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.
Combinatorial approach of in silico and in vitro evaluation of MLH1 variant associated with Lynch syndrome like metastatic colorectal cancer.
The testis-specific gene 1700102P08Rik is essential for male fertility.
Clinical significance of chromodomain helicase/ATPase DNA binding protein 1-like and human mutL homolog 1 gene expression in cholangiocarcinoma.
Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.
Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.
A highly malignant case of neuroblastoma with substantial increase of single-nucleotide variants and normal mismatch repair system: A case report.
Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells.
Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.
Sun J, Zhu L, Li Y, Tang J, Zhang T, Zhang H, Wang W, Li L, Bian S, Wu X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(4):e12670
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(4):e12670
DNA methylation mediates the immunosuppressive tumour microenvironment in metastatic endometrial clear cell carcinoma.
Jia H, Chen Y, Ma G, Xu S, Zhang X, Chang L, Yang P, Xiao Y, Xia X, Zhang S, Tang H, Mou Y, Zhang L, Wang H, Bai J, Yi X, Xing X
EBioMedicine 2025 Oct;120:105954
EBioMedicine 2025 Oct;120:105954
Somatic Kitl promotes mTOR to facilitate prophase I of meiosis in female embryonic gonads.
Liu C, Jin Z, Chen J, Li J, Feng G, Yin G, Yu Y, Ye X, Sun H, Zhang H, Gao F, Liu L
Cell death & disease 2025 Nov 17;16(1):838
Cell death & disease 2025 Nov 17;16(1):838
Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta.
Hatkevich T, Tezak BM, Acemel RD, Yee Chung VW, Lupiáñez DG, Capel B
Proceedings of the National Academy of Sciences of the United States of America 2025 Jan 7;122(1):e2413191121
Proceedings of the National Academy of Sciences of the United States of America 2025 Jan 7;122(1):e2413191121
Development of Syngeneic Murine Glioma Models with Somatic Mismatch Repair Deficiency to Study Therapeutic Responses to Alkylating Agents and Immunotherapy.
Bhatt D, Sundaram RK, López KSL, Lee T, Gueble SE, Vasquez JC
Current protocols 2025 Feb;5(2):e70097
Current protocols 2025 Feb;5(2):e70097
Defect of MLH1 expression sensitized esophageal squamous cell carcinoma cells to Polθ inhibitor.
Zhou B, Zhang M, Zhu C, Gao A, Su X, Guo M
Epigenomics 2025 Dec;17(18):1347-1354
Epigenomics 2025 Dec;17(18):1347-1354
Aberrant cytoplasmic localization of MLH1 characterizes a cell population that seeds breast cancer recurrence.
Mazumder A, Dewitt J, Oropeza E, Punturi N, Lozano D, Raghunathan M, Piscitelli J, Sajjadi E, GueriniRocco E, Venetis K, Ivanova M, Mane E, Dercole M, Concardi A, Fusco N, Manhart C, Bainbridge M, Haricharan S
Nature communications 2025 Dec 10;17(1):564
Nature communications 2025 Dec 10;17(1):564
Integrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models.
Xiying T, Ruxin GU, Jing T, Yu Z, RuiQian S, Gang Y, Shuo Z, Decai T
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2025 Apr;45(2):385-398
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2025 Apr;45(2):385-398
Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane.
Zhang X, Tan Y, Li T, Tan D, Fu B, Yang M, Chen Y, Cao M, Xuan C, Du Q, Hu R, Wang Q
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2024 Sep;76:101112
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2024 Sep;76:101112
The mechanism of RGS5 regulating gastric cancer mismatch repair protein.
Yang Z, Zhang R, Liu J, Tian S, Zhang H, Zeng L, Zhang Y, Gao L, Wang M, Shan W, Liu J
Molecular carcinogenesis 2024 Sep;63(9):1750-1767
Molecular carcinogenesis 2024 Sep;63(9):1750-1767
TDP43 Interacts with MLH1 and MSH6 Proteins in A DNA Damage-Inducible Manner.
Provasek VE, Kodavati M, Kim B, Mitra J, Hegde ML
Research square 2024 May 21;
Research square 2024 May 21;
TDP43 interacts with MLH1 and MSH6 proteins in a DNA damage-inducible manner.
Provasek VE, Kodavati M, Kim B, Mitra J, Hegde ML
Molecular brain 2024 Jun 5;17(1):32
Molecular brain 2024 Jun 5;17(1):32
Protein disulfide isomerase is essential for spermatogenesis in mice.
Zhang Y, Yang A, Zhao Z, Chen F, Yan X, Han Y, Wu D, Wu Y
JCI insight 2024 Jun 24;9(12)
JCI insight 2024 Jun 24;9(12)
Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types.
He Y, Lu F, Jiang C, Gong F, Wu Z, Ostrikov K
Scientific reports 2024 Feb 13;14(1):3599
Scientific reports 2024 Feb 13;14(1):3599
Maternal Obesity Induces the Meiotic Defects and Epigenetic Alterations During Fetal Oocyte Development.
Tang S, Wu H, Chen Q, Tang T, Li J, An H, Zhu S, Han L, Sun H, Ge J, Qian X, Wang X, Wang Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Aug;11(30):e2309184
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Aug;11(30):e2309184
Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer.
Jiang Y, Wang Y, Chen G, Sun F, Wu Q, Huang Q, Zeng D, Qiu W, Wang J, Yao Z, Liang B, Li S, Wu J, Huang N, Wang Y, Chen J, Zhai X, Huang L, Xu B, Yamamoto M, Tsukamoto T, Nomura S, Liao W, Shi M
Cell metabolism 2024 Aug 6;36(8):1806-1822.e11
Cell metabolism 2024 Aug 6;36(8):1806-1822.e11
Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination.
Chen S, Zhao L, Liu J, Han P, Jiang W, Liu Y, Hou J, Wang F, Li J
Cancer letters 2024 Aug 28;598:217105
Cancer letters 2024 Aug 28;598:217105
The MSH2 c.793-1G>A variant disrupts normal splicing and is associated with Lynch syndrome.
Li Y, Yu L, Cui J, Yin J, Wu W
Frontiers in oncology 2023;13:1131011
Frontiers in oncology 2023;13:1131011
Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small molecules.
Nguyen TP, Fang M, Kim J, Wang B, Lin E, Khivansara V, Barrows N, Rivera-Cancel G, Goralski M, Cervantes CL, Xie S, Peterson JM, Povedano JM, Antczak MI, Posner BA, Harvey CJB, Naughton BT, McFadden DG, Ready JM, De Brabander JK, Nijhawan D
Cell chemical biology 2023 Nov 16;30(11):1453-1467.e8
Cell chemical biology 2023 Nov 16;30(11):1453-1467.e8
Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small molecules.
Nguyen TP, Fang M, Kim J, Wang B, Lin E, Khivansara V, Barrows N, Rivera-Cancel G, Goralski M, Cervantes CL, Xie S, Peterson JM, Povedano JM, Antczak MI, Posner BA, McFadden DG, Ready JM, De Brabander JK, Nijhawan D
bioRxiv : the preprint server for biology 2023 Feb 21;
bioRxiv : the preprint server for biology 2023 Feb 21;
Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice.
Bai D, Zhu L, Jia Q, Duan X, Chen L, Wang X, Hou J, Jiang G, Yang S, Li S, Li XJ, Yin P
Progress in neurobiology 2023 Aug;227:102484
Progress in neurobiology 2023 Aug;227:102484
Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.
Ran X, Hu T, Li Z
Frontiers in oncology 2022;12:810631
Frontiers in oncology 2022;12:810631
NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.
Chen L, Wang WJ, Liu Q, Wu YK, Wu YW, Jiang Y, Liao XQ, Huang F, Li Y, Shen L, Yu C, Zhang SY, Yan LY, Qiao J, Sha QQ, Fan HY
Nucleic acids research 2022 Oct 28;50(19):10896-10913
Nucleic acids research 2022 Oct 28;50(19):10896-10913
Integrated proteogenomic characterization of urothelial carcinoma of the bladder.
Xu N, Yao Z, Shang G, Ye D, Wang H, Zhang H, Qu Y, Xu F, Wang Y, Qin Z, Zhu J, Zhang F, Feng J, Tian S, Liu Y, Zhao J, Hou J, Guo J, Hou Y, Ding C
Journal of hematology & oncology 2022 Jun 3;15(1):76
Journal of hematology & oncology 2022 Jun 3;15(1):76
Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.
Zhang XZ, Wei LL, Zhang XH, Jin HJ, Chen SR
Development (Cambridge, England) 2022 Jun 15;149(12)
Development (Cambridge, England) 2022 Jun 15;149(12)
The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.
Zhang XZ, Wei LL, Jin HJ, Zhang XH, Chen SR
Cell reports 2022 Jul 5;40(1):111049
Cell reports 2022 Jul 5;40(1):111049
Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.
Wu Q, Huang Q, Jiang Y, Sun F, Liang B, Wang J, Hu X, Sun M, Ma Z, Shi Y, Liang Y, Tan Y, Zeng D, Yao F, Xu X, Yao Z, Li S, Rong X, Huang N, Sun L, Liao W, Shi M
Cancer immunology research 2022 Feb;10(2):182-199
Cancer immunology research 2022 Feb;10(2):182-199
C. tropicalis promotes chemotherapy resistance in colon cancer through increasing lactate production to regulate the mismatch repair system.
Qu J, Sun Z, Peng C, Li D, Yan W, Xu Z, Hou Y, Shen S, Chen P, Wang T
International journal of biological sciences 2021;17(11):2756-2769
International journal of biological sciences 2021;17(11):2756-2769
Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.
Bai D, Yin P, Zhang Y, Sun F, Chen L, Lin L, Yan S, Li S, Li XJ
Human molecular genetics 2021 Jul 28;30(16):1497-1508
Human molecular genetics 2021 Jul 28;30(16):1497-1508
Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.
Li J, Liu F, Lv Y, Sun K, Zhao Y, Reilly J, Zhang Y, Tu J, Yu S, Liu X, Qin Y, Huang Y, Gao P, Jia D, Chen X, Han Y, Shu X, Luo D, Tang Z, Liu M
Nucleic acids research 2021 Feb 26;49(4):2027-2043
Nucleic acids research 2021 Feb 26;49(4):2027-2043
Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.
Zhu J, Ke G, Bi R, Wu X
Journal of surgical oncology 2020 Sep;122(3):538-546
Journal of surgical oncology 2020 Sep;122(3):538-546
Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.
Hu Z, Long T, Ma Y, Zhu J, Gao L, Zhong Y, Wang X, Wang X, Li Z
Journal of experimental & clinical cancer research : CR 2020 May 5;39(1):76
Journal of experimental & clinical cancer research : CR 2020 May 5;39(1):76
Combinatorial approach of in silico and in vitro evaluation of MLH1 variant associated with Lynch syndrome like metastatic colorectal cancer.
Saleem K, Zaib T, Ji W, Zhang C, Qin Q, Wang Y, Xu L, Yu H, Zhu S, Dong K, Si S, Jia X, Wu J, Fu S, Sun W
Bioscience reports 2020 Jun 26;40(6)
Bioscience reports 2020 Jun 26;40(6)
The testis-specific gene 1700102P08Rik is essential for male fertility.
Wu XL, Yun DM, Gao S, Liang AJ, Duan ZZ, Wang HS, Wang GS, Sun F
Molecular reproduction and development 2020 Feb;87(2):231-240
Molecular reproduction and development 2020 Feb;87(2):231-240
Clinical significance of chromodomain helicase/ATPase DNA binding protein 1-like and human mutL homolog 1 gene expression in cholangiocarcinoma.
Hua J, Li S, Huang C
Oncology letters 2018 Sep;16(3):2989-2994
Oncology letters 2018 Sep;16(3):2989-2994
Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.
Liang D, Shi S, Liang C, Meng Q, Zhang B, Ni Q, Xu J, Yu X
Surgery 2018 May;163(5):1080-1089
Surgery 2018 May;163(5):1080-1089
Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.
Yuan Y, Zhao Y, Chen L, Wu J, Chen G, Li S, Zou J, Chen R, Wang J, Jiang F, Tang Z
Nucleic acids research 2017 Sep 6;45(15):8676-8683
Nucleic acids research 2017 Sep 6;45(15):8676-8683
A highly malignant case of neuroblastoma with substantial increase of single-nucleotide variants and normal mismatch repair system: A case report.
Yuan LQ, Wang JH, Zhu K, Yang M, Gu WZ, Lai C, Li HM, Shu Q, Chen X
Medicine 2017 Dec;96(50):e8845
Medicine 2017 Dec;96(50):e8845
Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells.
He YQ, Sheng JQ, Ling XL, Fu L, Jin P, Yen L, Rao J
Experimental & molecular medicine 2012 Dec 31;44(12):723-32
Experimental & molecular medicine 2012 Dec 31;44(12):723-32
Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.
Jin P, Lu XJ, Sheng JQ, Fu L, Meng XM, Wang X, Shi TP, Li SR, Rao J
Cancer prevention research (Philadelphia, Pa.) 2010 Aug;3(8):910-6
Cancer prevention research (Philadelphia, Pa.) 2010 Aug;3(8):910-6
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11697-1-AP(MLH1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MLH1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MLH1. This antibody recognizes human,mouse,rat antigen. The MLH1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
