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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX101160 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101160, RRID:AB_1241059
- Product name
- MLH1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE GTX101160 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MLH1 antibody detects MLH1 protein by western blot analysis.A. 30 £gg A549 whole cell lysate/extract B. 30 £gg H1299 whole cell lysate/extract7.5 % SDS-PAGEMLH1 antibody (GTX101160) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MLH1 antibody detects MLH1 protein by western blot analysis. Non-transfected (-) and MLH1-transfected (+, ) 293T whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody (GTX101160) at a dilution of 1:20000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MLH1(GTX101160) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
