FNab05213

antibody from FineTest Biotech
Targeting: MLH1 COCA2, FCC2, HNPCC, HNPCC2
Provider product page for FNab05213
Western blot
ELISA
Immunocytochemistry
Immunoprecipitation
Immunohistochemistry

Antibody data

Product number
FNab05213 - Provider product page
Provider
FineTest Biotech
Product name
MLH1 antibody
Antibody type
Polyclonal
Antigen
Recombinant protein fragment
Description
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system(MMR). DNA repair is initiated by MutS alpha(MSH2-MSH6) or MutS beta(MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha(MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Conjugate
Unconjugated
Antigen sequence
MutL protein homolog 1
Epitope
IgG
Isotype
IgG
Vial size
100¦Ìg
Concentration
Immunogen affinity purified
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)
Handling
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)
MLH1 protein structure - FNab05213 shown in red.