Ab115549

antibody from Aladdin Scientific
Targeting: MLH1 COCA2, FCC2, HNPCC, HNPCC2
Provider product page for Ab115549

Antibody data

Product number
Ab115549 - Provider product page
Provider
Aladdin Scientific
Product name
Recombinant MLH1 Antibody
Antibody type
Recombinant
Description
Rabbit anti Human MLH1 Antibody, Recombinant, could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200 Protein FunctionHeterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Reactivity
Human
Host
Rabbit
Conjugate
Unconjugated
Antigen sequence
AA 350-756
Vial size
100_l,10_l,1ml,50_l
Concentration
0,6 mg/ml
Storage
Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
MLH1 protein structure - Ab115549 shown in red.