MA5-15431

antibody from Invitrogen Antibodies

Targeting: MLH1 COCA2, FCC2, HNPCC, HNPCC2

ELISA

Antibody data

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Validation data

Product number: MA5-15431 - Provider product page
Provider: Invitrogen Antibodies
Product name: MLH1 Monoclonal Antibody (4C9C7)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15431 targets MLH1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples. The MA5-15431 immunogen is purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.. MA5-15431 detects MLH1 which has a predicted molecular weight of approximately 85kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 4C9C7
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MLH1 protein structure - MA5-15431 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MLH1 using MLH1 monoclonal antibody (Product # MA5-15431) in HeLa (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS-7 (6) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of MLH1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042) (Assay ID CRISPR1027533_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of MLH1 was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa CAS9 (Lane 2), HeLa MLH1 KO (Lane 3) whole cell extracts. The blot was probed with Anti-MLH1 Monoclonal Antibody (4C9C7)(Product # MA5-15431) using 1:1000 dilution and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to MLH1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), PC-3 (Lane 2), MCF7 (Lane 3), A549 (Lane 4) and HCT 116 (Lane 5). The blot was probed with Anti-MLH1 Polyclonal Antibody (Product # MA5-15431, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 85 kDa band corresponding to MLH1 was observed across the cell lines tested except HCT 116.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MLH1 using MLH1 monoclonal antibody (Product # MA5-15431) in HeLa (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS-7 (6) cell lysate.